NucleoSpin DNA Stool (250)

Macherey-NagelSKU: MN-740472.250

Price:
$1,687

Description

Mini spin kit for isolation of DNA from stool samples, MN Bead Tubes Type A included

 

Specifications:

Application Isolation of DNA
Target DNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Package unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Handling Centrifugation
Lysate clarification Centrifugation, NucleoSpin Inhibitor Removal Column
Automated use No
Sample material Stool
Sample amount 180–220 mg
Fragment size 200 bp–approx. 50 kbp
Typical yield Depends on sample type, quality, and water content
Theoretical binding capacity 50 µg
Typical purity A260/A280 1.7–1.9
Elution volume 30–100 µL
Preparation time 60 min/10 preps
Typical downstream application Microarrays, PCR, Real-time PCR, Southern blotting
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Hazardous material Yes

 

SDS

Manual

 

Support Protocols

Chicken feces
Enrichment of Host DNA

 

Learn More

NucleoSpin DNA Stool

  • New technology for efficient removal of PCR inhibitors (like polysaccharides or bile salts in stool samples)
  • Lysis is supported by mechanical disruption with ceramic beads (MN Bead Tubes Type A)

 

NucleoSpin DNA Stool procedure
The very efficient, ceramic bead-based lysis procedure achieves unbiased mechanical lysis and makes the NucleoSpin DNA Stool kit ideal for microbiome studies.


Superior DNA yield and inhibition free amplification of the 16S rRNA gene
NucleoSpin DNA Stool quickly and reliably purifies high-quality microbial, host and pathogenic genomic DNA from various stool samples.

 

High genomic DNA yield, and purity from human stool samples

DNA was isolated from human stool samples with the NucleoSpin DNA Stool kit (MN) and with competitor products (MO, Z, Q). The DNA was extracted according to manufacturer’s protocols, and 5 % of the eluate was subjected to gel electrophoresis. DNA yield, and quality of the samples shown in (A) were assessed by means of UV absorption measurement. The genomic DNA isolated with the NucleoSpin DNA Stool kit showed superior yield, and quality.

 

Efficient removal of PCR inhibitors from various sample sources

 

DNA was isolated from human, and animal feces samples with the NucleoSpin DNA Stool kit.5 μL of undiluted eluate served as template for the amplification of a 1.5 kb fragment from the bacterial 16S rRNA gene in an endpoint-PCR (35 cycles). The DNA extracted with the NucleoSpin DNA Stool kit works undiluted in a PCR reaction, indicating the successful removal of PCR inhibitors.

 

Selected citations

Adamczyk et al. Plant roots increase both decomposition, and stable, organic matter formation in boreal forest soil. Nature Communications, volume 10, 3982 (2019).

Goncalves et al. Microbiota stimulation generates LCMV-specific memory CD8+ T cells in SPF mice, and determines their TCR repertoire during LCMV infection. Molecular Immunology, volume 124, pages 125–141 (2020).

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