NucleoSpin 8 Soil (12x8)

Macherey-NagelSKU: MN-740779

Price:
$516

Description

Isolation of total DNA from diverse soil types in flexible 8‑well strip format

 

Specifications:

Shelf life 39 Months
Application Isolation of DNA
Target DNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format 8-well strip
Package unit 96 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification Centrifugation, NucleoSpin Inhibitor Removal Strip
Automated use No
Sample material Sediment, Sludge, Soil
Sample amount < 500 mg
Fragment size 50 bp–approx. 50 kbp
Typical yield 2–10 µg (500 mg soil)
Theoretical binding capacity 50 µg
Typical purity A260/A280 1.6–1.8
Elution volume 100–200 µL
Preparation time 150 min/6 strips
Typical downstream application enzymatic reactions, Microarrays, PCR, qPCR
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Scope of delivery NucleoSpin Soil Binding Strips (12 x 8), MN Wash Plates, NucleoSpin Bead Tubes, NucleoSpin Inhibitor Removal Plates, buffers, MN Square-well Blocks, Racks of Tube Strips
Hazardous material Yes

 

SDS

Manual

 

Learn More

 
 

NucleoSpin 96 Soil

 

The NucleoSpin 96 Soil kit is optimized for parallel extraction of up to 96 samples. The kit can be used for a wide range of soil or sediment samples using either centrifugation or vacuum processing.

 

 

 
 

NucleoSpin 8 Soil


The NucleoSpin 8 Soil kit is optimized for parallel extraction of 8 to 48 samples.



NucleoSpin 96 Soil and NucleoSpin 8 Soil use mechanical disruption with ceramic MN Bead Tubes Type A in addition to highly specialized buffer combinations to ensure optimal lysis conditions.

 

NucleoSpin 96 Soil and NucleoSpin 8 Soil - Ideal for centrifuge or vacuum processing

 
Our NucleoSpin 96 Soil and NucleoSpin 8 Soil kits are ideal for convenient manual processing of up to 96 samples in parallel. Click here to learn more about processing of 96 well plates and 8 well strips with our NucleoVac 96 Vacuum Manifold.


 DNA purification from soil and peat 

 

 DNA isolation from Alaskan gelisol.

Bacterial genome copy numbers were determined for DNA that was extracted from 250 mg soil samples derived from active and permafrost layers of Alaska gelisol using the NucleoSpin 96 Soil kit in comparison with a competitor kit from MoBio. Far more bacterial DNA was extracted with the NucleoSpin 96 Soil kit using the combination of Buffer S1 and Buffer SX than with the competitor kit.

DNA isolation from spruce peat
Bacterial genome copy numbers were determined for DNA that was extracted from 500 mg samples of spruce peat using the NucleoSpin 96 Soil kit in comparison with a competitor kit. Far more bacterial DNA was extracted with the NucleoSpin 96 Soil kit using Buffer S1 (with or without SX) than with the competitor kit.

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