NucleoMag DNA Food (4x96)

Macherey-NagelSKU: MN-744945.4

Price:
$1,534

Description

NucleoMag® B-Beads, buffers, Proteinase K

Flexible magnetic bead based isolation of DNA from food and feed

Application Isolation of DNA
Target DNA
CE certified No, research use only
Technology Magnetic bead technology
Brand NucleoMag
Format Magnetic beads
Package unit 96 Prep(s), 384 Prep(s)
Handling Magnetic separation
Automated use Yes
Application note available with Thermo
Sample material Feed, Food
Sample amount < 200 mg
Fragment size 300 bp–approx. 50 kbp
Typical yield 0.1–10 µg (depending on sample type)
Theoretical binding capacity 0.4 µg/µL beads
Typical purity A260/A280 1.6–1.9
Elution volume 50–200 µL
Preparation time 40–120 min/96 preps (excl. lysis)
Typical downstream application enzymatic reactions, Real-time PCR, Southern blotting
Storage temperature 15−25 °C
Shelf life (from production) 24 Months
Hazardous material Yes

 

NucleoMag DNA Food

  • DNA extraction from food and feed for e.g., species identification, GMO detection
  • Get even low amounts of partially degraded DNA from complex matrices
  • Extraction of DNA from contaminating bacteria (food safety)
  • Kit chemistry allows full sample flexibility

NucleoMag DNA Food

The NucleoMag  DNA Food kit is designed for the isolation of DNA from food and feed samples, preferably of plant or animal origin. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Sample lysis is achieved by incubation of samples with Proteinase K at 65 °C. Lysis mixtures should be cleared by centrifugation or filtration in order to remove contaminants and residual cellular debris. For binding of nucleic acids to the paramagnetic beads, Binding Buffer CB and the NucleoMag B-Beads are added to the clear lysate. After magnetic separation, the paramagnetic beads are washed to remove contaminants and salts using Wash Buffers CMW, CQW, and 80 % ethanol. Residual ethanol from previous wash steps is removed by air drying. Next, highly purified DNA is eluted with low-salt Elution Buffer (CE) and can be used directly for downstream applications. The NucleoMag DNA Food kit can be used either manually, or automated on standard liquid handling instruments and automated magnetic separators.


Automate your DNA purification from food samples!

Automated purification of DNA from food and feed samples on the epMotion 5075t platform

MACHEREY-NAGEL and Eppendorf deliver a tailored solution for your high throughput DNA extraction from food and feed sample material. n Reliable performance and excellent DNA yields for e.g., species identification and GMO detection. n Excellent recovery from diverse and challenging food matrices. n Tailored protocol for processing variable sample number in multiples of 8 samples (8–96).

 

Automated isolation of DNA from maize and wheat samples


DNA was isolated from maize and wheat sample material (n = 8; 200 mg each sample) using the NucleoMag DNA Food kit on the epMotion 5075t platform. Total yield was determined by UV spectrometry (dark blue bars). A subsequent qPCR analysis was performed for a 103 bp actin amplicon using the SensiFastTM Probe Lo-ROX kit from BioLine on an Applied Biosystems 7500 Real-Time PCR System.

qPCR analysis of purified DNA from various food and feed samples


DNA was isolated from different food and feed samples (n = 4; 200 mg each sample) including raw meat, seeds, or shredded soybeans (dark blue bars) using the NucleoMag DNA Food kit the epMotion 5075t platform. A subsequent qPCR analysis was performed for a 103 bp actin amplicon using the SensiFastTM Probe Lo-ROX kit from BioLine on an Applied Biosystems 7500 Real-Time PCR System.

Integrity of genomic DNA from maize and wheat samples


DNA was isolated from maize and wheat sample material (n = 8; 200 mg each sample) using the NucleoMag DNA Food kit on the epMotion 5075t platform. The integrity was exemplarily analyzed by gel electrophoresis (1 μL per eluate; 1 % TAE gel; M: Lambda DNA/Hind III – Thermo Scientific).

 

Automated purification of genomic DNA from food samples on the HAMILTON NIMBUS Presto workstation

  • Save hands-on time by using automated plate-prefilling and plate-handling performed by the NIMBUS workstation
  • Benefit from the high-speed extraction procedure of the integrated KingFisher™ Presto unit n Efficient DNA isolation even from highly processed sample matrices
  • Reliable performance in downstream applications

Reliable DNA isolation from diverse food and feed samples


DNA was isolated from five different sample matrices (n=8 for each sample type), among them samples rich in polysaccharides and fats as well as highly processed materials. DNA was quantified photometrically. The isolation yielded high amounts of DNA from all samples materials tested (>10 µg DNA for all samples).

Reliable qPCR performance from diverse food and feed samples

DNA isolated from five different sample matrices (n=8 for each sample type) was used as input for a subsequent qPCR targeting a 103 bp actin amplicon. The qPCR was conducted using the SensiFast™ Probe Lo-ROX kit from BioLine on an Applied Biosystems 7500 Real-Time PCR System. The target was successfully amplified from all eluates tested.


Highly pure DNA from diverse food and feed samples


DNA was isolated from five different sample matrices (n=8 for each sample type), among them samples rich in polysaccharides and fats as well as highly processed materials. DNA purity was determined photometrically by measuring the OD ratios at 260/280 nm and 260/230 nm. All eluates exhibit values of >1.8 both for the 260/280 nm and 260/230 nm value.

 

Automated purification of DNA from food and feed samples on the Freedom EVO 150 platform

MACHEREY-NAGEL and TECAN deliver a tailored solution for your high throughput DNA extraction from food and feed sample material. We adapted the NucleoMag DNA Food procedure on the Freedom EVO 150 system to automate your nucleic acid purification workflow.

  • Reliable performance and excellent DNA yields for e.g., species identification and GMO detection
  • Excellent recovery from diverse and challenging food matrices
  • Tailored protocol for processing variable sample numbers

Purity of isolated DNA from food and feed samples


DNA was isolated from different food and feed samples (n = 4) using the NucleoMag DNA Food kit on the Freedom EVO 150 platform. Starting material was 50 mg/prep for rice or wheat, and 25 mg/prep for pig liver. The purity was determined by measuring A260/A280 (blue bars) and A260/A230 (orange squares) values via UV spectrometry

qPCR analysis of purified DNA from various food and feed samples


DNA was isolated from different food and feed samples (n = 4) including raw meat, seeds, or processed food (blue bars) using the NucleoMag DNA Food kit the Freedom EVO 150 platform. A subsequent qPCR analysis was performed for a 103 bp actin amplicon using the SensiFastTM Probe Lo-ROX kit from BioLine on an Applied Biosystems 7500 Real-Time PCR System.

qPCR performance analysis of purified nucleic acids from sausage samples


DNA was isolated from 50 mg of sausage samples using the NucleoMag DNA Food kit on a Freedom EVO 150 platform and subjected to a subsequent qPCR analysis using dilution series of the eluate (1:4 serial dilution). The qPCR was performed for a 103 bp actin amplicon using the SensiFastTM Probe Lo-ROX kit from BioLine on an Applied Biosystems 7500 Real-Time PCR System. The logarithms of the calculated eluate input volumes were plotted against the CT values (Sample - grey line) in comparison to a theoretical standard curve (standard - blue line) and the regression curve (linear sample - black line). The slope of the regression curve (-3.5246) and the calculated qPCR-efficiency (approx. 92,18 %) show an excellent qPCR-performance without PCR inhibition.

 

Automated DNA purification of food and feed samples on the Maelstrom 4800

Extraction of DNA from various food and feed samples 


Average yield from 200 mg per sample (28 mg for pig intestines) of samples extracted on the Maelstrom 4800 . DNA was eluted in 100 µL. A subsequent qPCR analysis was performed using an Actin amplicon to demonstrate the suitability for typical downstream analysis

DNA extracted from various food samples is reliably pure


Consistent purity levels (in terms of A260/A280 and A260/A230) indicate high reliability of NucleoMag DNA Food for purification of DNA on the Maelstrom 4800.

 

Automated purification of genomic DNA from food and feed samples on the KingFisher Flex platform

MACHEREY-NAGEL delivers a ready to use solution for your high throughput DNA extraction. We adapted the NucleoMag DNA Food procedure on instruments of the KingFisher series to speed up your nucleic acid purification workflow.

Reliable performance and excellent DNA yields for ,e.g., species identification and GMO detection

  • Speed up your DNA extraction by processing of 96 samples in less than 20 minutes (excluding lysis) 
  • Purchase the ultrafast KingFisher Flex platform and optimized MACHEREY-NAGEL extraction kits from one single source (one stop shopping**)

Extraction of DNA from various food and feed samples using different automation protocols


DNA was isolated from different food and feed samples including raw meat, seeds, or shredded soybeans (dark blue bars) using the NucleoMag DNA Food kit on a KingFisher Flex platform with the standard protocol. The performance of the fast protocol was compared to the equivalent standard protocol for soy material (orange bar). A subsequent qPCR analysis was performed for a 130 bp 18s rRNA amplicon using the Maxima SYBR Green kit from Thermo Scientific on an Applied Biosystems 7500 Real-Time PCR System.

qPCR performance analysis of purified nucleic acids from milk chocolate

DNA was isolated from 200 mg of grinded milk chocolate using the NucleoMag DNA Food kit on a KingFisher Flex platform and subjected to a subsequent qPCR analysis using different amounts of eluate (e.g., 1:4 serial dilution). The qPCR was performed for a 130 bp 18s rRNA amplicon using the Maxima SYBR Green kit from Thermo Scientific on an Applied Biosystems 7500 Real-Time PCR System. The logarithms of the calculated eluate input volumes were plotted against the CT values (blue) in comparison to a theoretical standard curve (grey) and the regression curve (black). The slope of the regression curve (-3.1968) and the calculated qPCR-efficiency (approx. 105 %) show an excellent qPCR-performance without PCR inhibition.



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