NucleoBond PC 10000 (5)

Macherey-NagelSKU: MN-740593

Price:
$638

Description

1st generation anion exchanger for purification of plasmid DNA

 

Specifications:

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Giga prep
Package unit 5 Prep(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 500−2000 mL (high copy), 1−4 L mL (low copy)
Vector size < 300 kbp
Typical yield 2000−10000 µg
Theoretical binding capacity 10000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 120−150 min/2 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Scope of delivery NucleoBond AX 10000 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Hazardous material Yes

 

SDS

Manual

Supplementary Note

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