NucleoBond HMW DNA (20)

Macherey-NagelSKU: MN-740160.20

Price:
$254

Description

Anion exchange chromatography for purification of high integrity DNA from different sample materials

 

Specifications:

Shelf life 39 Months
Application Isolation of DNA
Target DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Package unit 2 Prep(s), 20 Prep(s)
Handling Gravity flow
Automated use No
Sample material Animal tissue, Bacteria, Blood, Body fluids, Cells, Human tissue, Plant, Yeast
Sample amount < 300 mg human/animal tissue, < 10⁷ cultured cells, < 1.5 g plant leaves, < 30 mg bacteria/yeast (ground under liquid nitrogen), < 300 mg bacteria/yeast (lysed enzymatically or by bead beating), < 2 mL liquid sample
Fragment size 2 kbp–approx. 150 kbp (enzymatic lysis)/50 kbp (mechanical lysis)
Typical yield Depending on amount and quality of sample
Theoretical binding capacity 600 µg
Typical purity A260/A280 1.8–1.9
Elution volume 50–250 µL
Preparation time 2 h/12 preps (incl. 30 min lysis)
Typical downstream application Long-read sequencing, Next Generation Sequencing
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes

 

SDS

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