NucleoBond Finalizer Large (20)

Macherey-NagelSKU: MN-740418.20

Price:
$89

Description

Proven syringe filter for speeding up anion exchange plasmid preparations

 

Specifications:

Shelf life 39 Months
Application Concentration and desalting of plasmid eluates
Target Plasmid DNA
CE certified No, research use only
Technology Filtration
Brand NucleoBond
Format Syringe filter large
Package unit 20 Set(s)
Handling Manual
Automated use No
Sample material Bacteria, E. coli
Sample amount 15 mL DNA eluate
Vector size 2−50 kbp
Typical recovery 60−90 %
Theoretical binding capacity 2000 µg
Residual cloride concentration < 0.3 µg/µl
Elution volume 400−1000 µL
Preparation time 5 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 24 Months
Hazardous material No

 

Manual

 

Learn More


Plasmid DNA - Desalting and Concentraction

Following the elution of pure plasmid DNA from the NucleoBond columns, MN provides a range of options for desalting and concentrating the plasmid. In addition to the standard isopropanol precipitation followed by centrifugation we offer the NucleoBond Finalizers for manaul desalting with a syringe as well as centrifuge-compartible NucleoSPin Finisher and vacuum compartible NucleoSnap Finisher for a fast, simple and easy prep.

 


NucleoBond Finalizer
The NucleoBond Finalizers are designed to concentrate and desalinate plasmid DNA eluates collected using NucleoBond columns after anion-exchange chromatographic DNA purification. The sample is syringe-loaded onto a NucleoBond Finalizer membrane to avoid tedious centrifugation steps.


NucleoSpin and NucleoSnap Finisher
NucleoSpin Finisher are designed for quick concentration and desalting of plasmid eluates obtained by anion-exchange chromatography. Finisher replace tedious and time-consuming isopropanol precipitation. Furthermore, problems like lost DNA pellets or incomplete solubilization of hardly visible precipitates can easily be circumvented.


NucleoMag Desalting Beads
NucleoMag Desalting beads are designed for quick concentration and desalination of plasmid DNA eluates that are obtained be anion exchange chromatographic plasmid purification kits. Plasmid DNA is bound reversibly to NucleoMag Desalting beads followed by ethanolic wash steps. Finally pure plasmid DNA is eluted using a low salt buffer. NucleoMag Desalting beads can be automated on e.g the Andrew+ platform

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