Description
NextPrep-Mag™ Urine cfDNA Isolation Kit
- Ideal for extracting DNA from urine for cell-free DNA analysis
- Generates highly concentrated libraries using few PCR cycles
- Automation friendly, mag-bead based format
- Fast, 30 minute protocol with minimal hands-on time
- Ideal for NGS library prep
- No vacuum pumps, vacuum manifolds, or column extenders required
Extracting cfDNA from Urine
The NextPrep-Mag™ Urine cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) for constructing NGS libraries from urine, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cell free DNA is ideal for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the NEXTFLEX® Cell Free DNA-Seq Library Prep Kit for Illumina® Sequencing and the NEXTFLEX® Cell Free DNA Library Prep Kit for Ion Torrent™ Sequencing.
NextPrep-Mag™ Urine cfDNA Isolation Kit Compared to the QIAamp™ Circulating Nucleic Acid Kit
The NextPrep-Mag™ Urine cfDNA Isolation Kit offers a number of advantages over the QIAamp™ Circulating Nucleic Acid Kit, including speed of cfDNA isolation and ease of automation, while delivering comparable cfDNA yields and higher concentration NGS libraries.
KIT CONTENTS
- cfDNA DNA Proteinase K
- Urine cfDNA Binding Solution
- cfDNA Magnetic beads
- cfDNA Wash Solution 1
- cfDNA Wash Solution 2
- cfDNA Elution Solution
- Control PCR Primers
REQUIRED MATERIALS NOT PROVIDED
- Ethanol, 100%
- Vortex mixer
- Heat block at 55°C
- Magnetic stands to hold 2 mL microcentrifuge tubes and 15 mL or 50 mL tubes
- Microcentrifuge or low-speed “picofuge”
- Nuclease-free 2 mL (1.5 mL and 0.5 mL optional) microcentrifuge tubes and 15 mL tubes
- Pipettors (P-1000, P-200, P-20) and tips
- Disposable pipettes (variable sizes depending on sample volume processed)
Kit Specifications:
The NextPrep-Mag™ Urine cfDNA Isolation Kit For < 1 mL – 4 mL contains sufficient reagents for extraction of DNA from 50 samples of 1 mL urine or less, or 12 samples of 4 mL of urine. The NextPrep-Mag™ Urine cfDNA Isolation Kit For 4 mL – > 20 mL contains sufficient reagents for extraction of DNA from 25 samples of 4 mL urine or less, or 5 samples of 20 mL of urine. The kit components are stable for at least 12 months from time of receipt, when properly stored.