The NextPrep-Mag™ cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) for constructing NGS libraries from human blood plasma or serum, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cell free DNA is ideal for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the Bioo Scientific NEXTflex™ Cell Free DNA-Seq Library Prep Kit for Illumina Sequencing and the NEXTflex Cell Free DNA Library Prep Kit for Ion Proton and Ion PGM Sequencing. This kit is optimized for isolating circulating tumor and cell free circulating fetal DNA from blood plasma.
The NextPrep-Mag cfDNA Isolation Kit For 1 - 3 mL Plasma Samples contains sufficient reagents for extraction of DNA from 50 samples of 1 mL plasma, or 16 samples of 3 mL plasma. The NextPrep-Mag cfDNA Isolation Kit For 3 - 5 mL Plasma Samples contains sufficient reagents for extraction of DNA from 50 samples of plasma ranging from 3 mL to 5mL. The kit components are stable for at least 12 months from time of receipt, when properly stored.