- Indexed adapters compatible with Illumina® platforms for multiplexing libraries
- Up to 48 unique adapters available
- Considerably reduce your per-sample sequencing cost by multiplexing libraries
- Increase your sequencing scale by pooling 100s of samples on a single flow cell
- Validated on Illumina® next-generation sequencing platforms
- Compatible with the NEXTFLEX® Rapid RNA-Seq Kit and the NEXTFLEX® Rapid Directional RNA-Seq Kit
- Not compatible with the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Added Flexibility to Library Preparation
The NEXTFLEX® RNA-Seq Barcodes are indexed adapters that are designed for use with the NEXTFLEX® Rapid RNA-Seq Kit and the NEXTFLEX® Rapid Directional RNA-Seq Kit. These barcodes are not recommended for use with the new NEXTFLEX® Rapid Directional RNA-seq kit 2.0.
These indexes are composed of a 6 nt error-correcting sequence, offering an improved multiplex workflow and adding flexibility to library prep. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. Their automation-friendly format enables multiplexing of up to 48 samples. The NEXTFLEX® RNA-Seq Barcodes are available in sets of 6, 12, 24 and 48 unique adapters. We also offer the NEXTFLEX-96™ RNA-Seq Barcode Kits, which contain 96 – 8 nt index barcodes in a 96-well format (cat # NOVA-512915) .
Avoiding Registration Failure with Low Level Multiplexing
Registration failure could occur if the color balance was not maintained between the red and green lasers (used to sequence A/C bases and G/T bases, respectively). Read our blog post, Tech Tips – Barcode Recommendations for Low Level Multiplexing, to learn how to avoid registration failure on an Illumina® sequencer caused by lack of sufficient index sequence diversity.
- NEXTFLEX® RNA-Seq Barcode Adapter (0.6 µM)
- NEXTFLEX® Primer Mix (12.5 µM)
Sequences of NEXTFLEX RNA-Seq Barcode Indexes
Sequences of NEXTFLEX-96 RNA-Seq Barcode Indexes
Which kits can the NEXTFLEX® RNA-Seq Barcodes be used with?
The NEXTFLEX® RNA-Seq Barcodes were designed to be used with the NEXTFLEX® Rapid Directional RNA-Seq Kit and the NEXTFLEX® Rapid RNA-Seq Kit; however, they are compatible with other Illumina® RNA-seq library prep methods.
What concentration are the NEXTFLEX® RNA-Seq Barcodes and the NEXTFLEX-96™ RNA-Seq Barcodes supplied at?
They are supplied at a 0.6 µM concentration.
Are the NEXTFLEX® RNA-Seq Barcodes supplied with PCR primers?
Yes they are. If you need additional PCR primers they are available in 48, 96, 100, 192, 384 and 768 reaction sizes and the cost is $1/reaction. These are supplied at a concentration of 12.5 µM.
Are the sequences of the indexes used in the NEXTFLEX® RNA-Seq Barcodes available?
Yes, a PDF containing the indexes within the NEXTFLEX® RNA-Seq Barcodes can be downloaded here. Additionally, Excel files containing the index sequences are also available upon request.
The NEXTFLEX® RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique Illumina-compatible adapters, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice.