Reliable: Consistent yields and high-quality sequencing metrics
Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads
Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time
Flexible: Chemistry optimized with NEXTFLEX® color-balanced barcodes that allow a wide range of multiplexing (from 2 up to 384 samples in one run)
Efficient: Automated on the PerkinElmer Sciclone® G3 and Zephyr® G3 NGS workstation
Streamlined Enzymatic Fragmentation and Library Prep Workflow for Illumina® Sequencing
The NEXTFLEX® Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTFLEX® barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina® sequencing.
Figure 1: Reliable performance of NEXTFLEX® Rapid XP DNA-seq fragmentation enzyme. DNA is fragmented more evenly and into smaller sizes using the NEXTFLEX® Rapid XP DNA-seq kit than Competitor K’s kit. Each dot indicates each library peak size. (A) Libraries were prepared from 1 µg of DNA with 7 minutes of fragmentation with 2 PCR cycles: NEXTFLEX® Rapid XP DNA-seq kit (484 ± 52 bp, 11 %, n=17), Competitor K’s kit (1266 ± 451 bp, 36 %, n=17). (B) Libraries were prepared from 100 ng of DNA with 8 minutes of fragmentation with 5 PCR cycles: NEXTFLEX® Rapid XP DNA-seq kit (457 ± 12 bp, 3 %, n=18), Competitor K’s kit (730 ± 119 bp, 16 %, n=17). (C) Libraries were prepared from 1 ng of DNA with 15 minutes of fragmentation with 12 PCR cycles: NEXTFLEX® Rapid XP DNA-seq kit (448 ± 54 bp, 12 %, n=17), Competitor K’s kit (628 ± 160 bp, 25 %, n=17). All different input libraries were generated from 6 different commercially available DNA samples. (mean ± standard deviation, coefficient of variation)
Flexible Multiplexing Options
We offer a broad range of NEXTFLEX® adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTFLEX® Rapid XP DNA-seq kit is compatible with NEXTFLEX® DNA Barcodes, NEXTFLEX-96™ DNA Barcodes, NEXTFLEX® ChIP-Seq Barcodes, and NEXTFLEX-96™ ChIP-Seq Barcodes, and and NEXTFLEX-HT™ barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTFLEX® Unique Dual Index Barcodes and the NEXTFLEX® Dual-Indexed barcodes.
For more information about barcode options compatible with the NEXTFLEX® Rapid XP DNA-seq kit, please contact email@example.com
The NEXTFLEX® Rapid XP DNA-seq kit is designed to function with multiple liquid handlers, including the PerkinElmer Sciclone® and the Zephyr® G3 NGS workstations. For more information, or to obtain a program, contact firstname.lastname@example.org.
For availability of custom format and bulk packaging for larger volume requirements, please contact email@example.com for further information.
Size selection is a critical issue in NGS library preparation. Our blog post, Tech Tips – When to Size Select, addresses a number of factors to consider before determining a size selection strategy.
For research use only. Not for use in diagnostic procedures.Tech Tips – When to Size Select