NEXTflex™ Rapid RNA-Seq Kit

PerkinElmerSKU: NOVA-5138-02

Title: 48 rxn


  • Faster than traditional Illumina® RNA library prep protocols
  • Complete solution includes thermostable NEXTFLEX® Rapid Reverse Transcriptase
  • Input – 10 ng – 1 µg total RNA for enrichment by NEXTFLEX® Poly(A) Beads 2.0 or ~ 1 ng – 100 ng isolated mRNA or rRNA-depleted RNA
  • 96 barcodes are available for multiplexing
  • Automation protocol is available for the Sciclone® NGSx workstation
  • Functionally validated with Illumina® sequencing platforms


Automated RNA Library Prep Protocols

An optimized automation protocol for RNA library prep using the NEXTFLEX® Rapid RNA-Seq Library Prep Kit are available for the Sciclone® NGSx workstations is now available. Download the Sciclone® NGS and NGSx Workstation Automation Guide for the NEXTFLEX® Rapid RNA-Seq Kit.


Magnetic Beads for mRNA Purification

The NEXTFLEX® Poly(A) Beads 2.0 now provide a convenient method for batch purification of pure, intact mRNA upstream NEXTFLEX® Rapid RNA-Seq library preparation. NEXTFLEX® Poly(A) Beads 2.0 use oligo(dT) primer to isolate polyadenylated messenger RNAs from 10 ng – 5 µg of previously isolated total RNA.



  • NEXTFLEX® RNA Fragmentation Buffer
  • NEXTFLEX® First Strand Synthesis Primer
  • NEXTFLEX® First Strand Synthesis Buffer Mix
  • NEXTFLEX® Rapid Reverse Transcriptase
  • NEXTFLEX® Second Strand Synthesis Mix
  • NEXTFLEX® Adenylation Mix
  • NEXTFLEX® Ligation Mix
  • NEXTFLEX® RNA-Seq Barcode Adapter 1 (0.6 μM)
  • NEXTFLEX® Primer Mix (12.5 μM)
  • NEXTFLEX® PCR Master Mix
  • Nuclease-free Water
  • Resuspension Buffer



  • 10 ng – 1 µg total RNA for enrichment by NEXTFLEX® Poly(A) Beads 2.0 or ~ 1 ng – 100 ng isolated mRNA or rRNA-depleted RNA
  • DynaMag-2 Magnet (Life Technologies Cat # 123-21D)
  • Commercial kits for rRNA depletion (optional, if not using Poly(A) enrichment)
  • 100% Ethanol (stored at room temperature)
  • 80% Ethanol (stored at room temperature)
  • 2, 10, 20, 200 and 1000 μL pipettes
  • RNase-free pipette tips
  • Nuclease-free 1.5 mL microcentrifuge tubes
  • Thin wall nuclease-free 0.5 mL microcentrifuge tubes
  • 96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
  • Adhesive PCR Plate Seal (Bio-Rad, Cat # MSB1001)
  • Agencourt AMPure XP 60 mL (Beckman Coulter Genomics, Cat # A63881)
  • Magnetic Stand -96 (Thermo Fisher®, Cat # AM10027) or similar for post PCR cleanup
  • Microcentrifuge
  • Thermocycler
  • Heat block
  • Vortex



The NEXTFLEX® Rapid RNA-Seq Illumina® library prep kit contains enough material to prepare 8 or 48 RNA samples for RNA seuencing on an Illumina® platform. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at -20°C.



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