NEXTflex™ Dual-Indexed DNA Barcodes (1-96)

PerkinElmerSKU: NOVA-514160



  • Enables deep multiplexing of Illumina® DNA libraries for NGS applications
  • 8 nt index contained within adapter sequence
  • Increase your sequencing scale by pooling up to 192 samples on a single flow cell
  • The kits are supplied in a 96-well plate format
  • Compatible with Illumina® next-generation sequencing platforms


Increase Scale while Reducing Cost

The NEXTFLEX® Dual-Indexed DNA Barcodes are two kits that each contain 96 combinatorial dual indexed barcoded adapters for Illumina® library prep which enable deep multiplexing of NGS sequencing samples compatible with high-throughput automation. They significantly increase scale, while reducing costs by allowing the user to pool up to 192 samples in a single flow cell.


Multiplexing Options

Libraries made using the NEXTFLEX® Dual-Indexed DNA Barcodes can be combined with single index libraries using a dual index sequencing read primer. The NEXTFLEX® Dual-Indexed DNA Barcodes can be used with single, paired-end and multiplex reads, and are compatible with the NEXTFLEX® Rapid DNA-Seq Kit 2.0, the NEXTFLEX® Methyl DNA-Seq Kit, the NEXTFLEX® Cell Free DNA-Seq Kit and other genomic DNA library prep kits.


Kit Contents:

  • NNEXTFLEX® Dual-Indexed Adapter (25 µM)
  • NEXTFLEX® Primer Mix (12.5 µM)



Sequences of NEXTflex Dual-Indexed DNA Barcode Indexes



The NEXTFLEX® Dual-Indexed DNA Barcodes contain 8 reactions each of 96 unique adapters compatible with Illumina® platforms, enabling the user to multiplex up to 192 samples. This kit is shipped on dry ice.

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