NEXTflex™ ChIP-Seq Kit

PerkinElmerSKU: NOVA-5143-02

Title: NOVA-5143-02
Price:
$2,172.50

Description

  • Optimized for down to 1 ng of DNA input
  • For use with ChIP or genomic DNA samples
  • Optimized for low DNA input
  • Enhanced Adapter Ligation Technology offers optimal coverage and unique reads
  • Flexible barcode options – up to 96 unique, single-index barcoded adapters or 1,536 UDI barcodes available
  • Compatible with the Illumina® MiSeq®, HiSeq®, HiSeq®, HiSeq® X Ten, and NextSeq® 500 sequencing platforms

 

Designed for Ultra-low DNA Input

The NEXTFLEX® ChIP-Seq Kit is a library prep kit specifically designed for ultra-low DNA input Illumina® sequencing applications. As little as 1 ng of chromatin immunoprecipitated or genomic DNA is required to produce viable sequencing libraries compatible with single-read or paired-end sequencing.

The NEXTFLEX® ChIP-Seq Kit also features proprietary Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads and increased yields from minimal quantities of starting material. The NEXTFLEX® ChIP-Seq Kit now incorporates a bead-based size selection protocol, which completely eliminates the gel purification step. Additionally, there is an optional protocol which eliminates the size selection step entirely.

 

Unparalleled Multiplexing Capabilities

Using the NEXTFLEX® ChIP-Seq Barcodes, the user can index 6, 12, 24, 48, or even 96 samples at once providing unparalleled Illumina® ChIP sequencing capacity.

 

Kit Contents:

  • NEXTFLEX® ChIP End Repair Buffer Mix
  • NEXTFLEX® ChIP End Repair Enzyme Mix
  • NEXTFLEX® ChIP Adenylation Mix
  • NEXTFLEX® ChIP Ligation Mix
  • NEXTFLEX® ChIP Adapter (0.6 µM)
  • NEXTFLEX® ChIP Primer Mix (12.5 µM)
  • NEXTFLEX® ChIP PCR Master Mix
  • Nuclease-free Water
  • Resuspension Buffer
  • ChIP-Seq DNA Control

REQUIRED MATERIALS NOT PROVIDED

  • 1 ng – 10 ng of DNA in up to 40 µL nuclease-free water
  • NEXTFLEX® ChIP-Seq Barcodes – 6 / 12 / 24 / 48
  • Ethanol 100% (room temperature)
  • Ethanol 80% (room temperature)
  • Covaris System (S2, E210) or other device for fragmenting DNA
  • 96 well PCR Plate Non-skirted (Phenix Research®, Cat # MPS-499) / or / similar
  • 96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) / or / similar
  • Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001)
  • Agencourt® AMPure® XP 5 mL (Beckman Coulter Genomics®, Cat # A63880)
  • Magnetic Stand -96 (Thermo Fisher® Scientific, Cat # AM10027) / or / similar
  • Heat block
  • Thermocycler
  • 2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
  • Nuclease-free barrier pipette tips
  • Microcentrifuge
  • 1.5 mL nuclease-free microcentrifuge tubes
  • Vortex

 

Kit Specifications:

The NEXTFLEX® ChIP-Seq Library Prep Kit contains enough material to prepare 8 or 48 ChIP or genomic DNA samples for Illumina® sequencing. The shelf life of all reagents is 12 months when stored properly. This kit is shipped on dry ice.

 

Manual

Sheet

Tips

Data

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