The QuantSeq REV Kit is an all-in-one library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
With this kit the exact 3’ end can be pinpointed as the NGS reads directly start from the poly(A) tail using a Custom Sequencing Primer (CSP) which is included in the kit. This version of the kit is also recommended for paired-end sequencing.
For multiplexing of up to 384 samples/lane, the Lexogen i5 6 nt Dual Indexing Add-on Kit (Cat. No. 047) can be used that allows the dual indexing with four additional i5 indices. Alternatively, up to 384 pre-mixed UDIs of the Lexogen UDI 12 nt Unique Dual Indexing Add-on Kits (Cat. No. 107-111.96 and 120.384) can be used to replace the i7 6 nt indices provided in the QuantSeq REV kits.
NOTE! QuantSeq Kits are provided with free access to the BlueBee Data Analysis Pipeline. Before starting your library prep, please check the online Frequently Asked Questions (FAQ 1.28) to see if your species is available on the BlueBee Platform. Lexogen can add reference genomes for new species to the BlueBee Data Analysis Pipeline upon request. Please note this will incur a fee.
New reference genome implementation timelines are dynamic due to feasibility checks and formatting adjustments and therefore cannot be precisely guaranteed. We recommend submitting your request as early as possible prior to sequencing your libraries. New genome upload acceptance is also subject to change in the case of compatibility issues.
The QuantSeq data analysis pipeline is available on the OnRamp Platform ROSALIND, which allows Lexogen’s customers to analyze, interpret and collaborate globally on differential gene expression analysis without the need for specialized bioinformatics or programming skills. Every Lexogen’s customer purchasing a QuantSeq kit (FWD or REV) before July 1, 2021 is eligible for 24 free runs on ROSALIND.
Features and Application:
- Gene expression studies
- 3’ UTR tagging
- Alternative polyadenylation
- eQTL analysis
- Suitable for low quality RNA (including FFPE samples)
- From as low as 10 ng total RNA
- Reagent Box
- Lexogen i7 6 nt Index Set (formerly named: i7 Index Plate) with i7 indices 7001-7024 or 7001-7096 depending on the kit size (24, 96)
- Purification Module with Magnetic Beads
- HiSeq 2000/2500/3000/4000
- NextSeq 500/550 (with CSP Version 5)
- NovaSeq 6000
- Genome Analyzer