Description
This mix provides a single-tube solution for library construction for Illumina platforms. The protocol supports fragmentation, end-repair and dA-tailing in a single reaction step, therefore greatly simplifying the workflow, reducing the total reaction time and hands-on time.
PRODUCT SPECIFICATION*
| Storage Temperature | -25⁰C to -15⁰C |
|---|
| Assay | DNA Library Construction Assay |
|---|---|
| Specification | Functional |
* For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section.
QUALITY CONTROL ANALYSIS
The functionality of the 5X WGS Fragmentation Mix is evaluated by performing library construction, PCR/qPCR and sequencing.
Supplied with:
B0330 10X Fragmentation Buffer
B0340 Enhancer
NOTES
Enzyme components were tested prior to assembly and found free of contaminating endonucleases and exonucleases. Enzyme purity was >95% as determined by SDS-PAGE and negligible E.coli genomic DNA contamination was confirmed by qPCR.
LEGAL DISCLAIMER
For Research Use Only. Not for use in diagnostic procedures.
Limitations of Use
This product was developed, manufactured, and sold for in vitro use only. The product is not suitable for administration to humans or animals. SDS sheets relevant to this product are available upon request.