5X ER/A Tailing is a NGS library preparation module that uses a one-step reaction to combine end-repair and dA-tailing to convert fragmented DNA into 5′-phosphorylated and 3′-dA-tailed DNA fragments enabling direct ligation of Illumina sequencing adapters. When used in combination with the 5X WGS ligation module (L6030-W-L), the optimized chemistry ensures high sensitivity for low input DNA, high ligation efficiency for maximum library yield and a workflow that is under 3 hours with less than 45 minutes hands on time.
|Storage Temperature||-25° to -15°C|
|Assay||ER/A Tailing Enzme Mix Functional Assay|
* For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section.
QUALITY CONTROL ANALYSIS
ER/A Tailing Enzyme Mix Functional Assay: QC Library length must be within 15% of the reference library length. Concentration of the QC library generated from 100 ng input DNA (average ~300 bp fragments) is >60 nm with mapped reads > 90%. For QC library, normalized coverage should be within 0.7 to 1.3 for most of the genome (10% – 80% GC content).
Supplied with: B9420 10x ERA Buffer
Enzyme components were tested prior to assembly and free of contaminating endonucleases and exonucleases. Enzyme purity was >95% as determined by SDS-PAGE and negligible E. coli genomic DNA contamination was confirmed by qPCR.
For Research Use Only. Not for use in diagnostic procedures.
Limitations of Use
This product was developed, manufactured, and sold for in vitro use only. The product is not suitable for administration to humans or animals. SDS sheets relevant to this product are available upon request.