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This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCrystal clear seal specifically developed for optical applications, particularly qPCR\u003c\/li\u003e\n\u003cli\u003eIt is non sticky when removed from the packaging; this aids handling when wearing gloves\u003c\/li\u003e\n\u003cli\u003eThe adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal\u003c\/li\u003e\n\u003cli\u003eWhen the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear\u003c\/li\u003e\n\u003cli\u003eFor all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eApplications: qPCR and other fluorescent applications, plate readers, microscopy and protein crystallization (96 or 384 well)\u003c\/li\u003e\n\u003cli\u003eRemoval: will not leave a sticky residue on the plate surface following removal\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNon-pierceable\u003c\/li\u003e\n\u003cli\u003eCrystal Clear\u003c\/li\u003e\n\u003cli\u003ePeelable\u003c\/li\u003e\n\u003cli\u003eSeal integrity range: -40 °C to 121 °C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eContents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e IST-130-080LS                 100 Sheets, 140mm x 80mm\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible Equipments\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with 80mm Rolls –\u003c\/strong\u003e KAPS 500 Auto Sealer\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with Sheets –\u003c\/strong\u003e QuickSeal Hand Roller\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"iST Scientific","offers":[{"title":"Default Title","offer_id":39663904620588,"sku":"IST-130-080LS","price":226.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/products\/ANGLE-IST-130_b56d6b3b-e0a7-437f-bb8b-16fd9036b88b.png?v=1637722408"},{"product_id":"quickseal-qpcr-crystal-ultra-sterile-15725mmx80mm","title":"QuickSeal qPCR Crystal Ultra - Sterile   Roll   100M x 80mm","description":"\u003cp\u003eCrystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCrystal clear seal specifically developed for optical applications, particularly qPCR\u003c\/li\u003e\n\u003cli\u003eIt is non sticky when removed from the packaging; this aids handling when wearing gloves\u003c\/li\u003e\n\u003cli\u003eThe adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal\u003c\/li\u003e\n\u003cli\u003eWhen the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear\u003c\/li\u003e\n\u003cli\u003eFor all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eApplications: qPCR and other fluorescent applications, plate readers, microscopy and protein crystallization (96 or 384 well)\u003c\/li\u003e\n\u003cli\u003eRemoval: will not leave a sticky residue on the plate surface following removal\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNon-pierceable\u003c\/li\u003e\n\u003cli\u003eCrystal Clear\u003c\/li\u003e\n\u003cli\u003ePeelable\u003c\/li\u003e\n\u003cli\u003eSeal integrity range: -40 °C to 121 °C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eContents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e IST-130-080SR                 1 Roll, 100M x 80mm\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible Equipments\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with 80mm Rolls –\u003c\/strong\u003e KAPS 500 Auto Sealer\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with Sheets –\u003c\/strong\u003e QuickSeal Hand Roller\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"iST Scientific","offers":[{"title":"Default Title","offer_id":39663904653356,"sku":"IST-130-080SR","price":2063.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/products\/ANGLE-IST-130_45593507-65a3-49f6-9960-574f952cab60.png?v=1637722398"},{"product_id":"quickseal-qpcr-crystal-ultra-sterile-15825mmx80mm","title":"QuickSeal qPCR Crystal Ultra - Sterile   Pk of 100 Sheets   140mm x 80mm","description":"\u003cp\u003eCrystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCrystal clear seal specifically developed for optical applications, particularly qPCR\u003c\/li\u003e\n\u003cli\u003eIt is non sticky when removed from the packaging; this aids handling when wearing gloves\u003c\/li\u003e\n\u003cli\u003eThe adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal\u003c\/li\u003e\n\u003cli\u003eWhen the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear\u003c\/li\u003e\n\u003cli\u003eFor all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eApplications: qPCR and other fluorescent applications, plate readers, microscopy and protein crystallization (96 or 384 well)\u003c\/li\u003e\n\u003cli\u003eRemoval: will not leave a sticky residue on the plate surface following removal\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNon-pierceable\u003c\/li\u003e\n\u003cli\u003eCrystal Clear\u003c\/li\u003e\n\u003cli\u003ePeelable\u003c\/li\u003e\n\u003cli\u003eSeal integrity range: -40 °C to 121 °C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eContents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e IST-130-080SS                 100 Sheets, 140mm x 80mm\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible Equipments\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with 80mm Rolls –\u003c\/strong\u003e KAPS 500 Auto Sealer\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with Sheets –\u003c\/strong\u003e QuickSeal Hand Roller\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"iST Scientific","offers":[{"title":"Default Title","offer_id":39663904686124,"sku":"IST-130-080SS","price":272.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/products\/ANGLE-IST-130_e28ae83f-417f-4feb-b4ad-c218b8f54302.png?v=1637722385"},{"product_id":"ampfi-2x-qpcr-mastermix","title":"AmpFi 2x qPCR MasterMix","description":"\u003cp\u003eAmpFi 2x qPCR MasterMix provides analysis of DNA samples for quantitative real-time in a convenient, reliable setup .\u003c\/p\u003e\n\u003cp\u003eThis qPCR MasterMix contains Taq Polymerase, DNA Polymerase, providing for rapid extension rates. This polymerase provides increased yields, and sensitivity, while optimizing reaction times by up to 70%, compared to wild-type Taq DNA polymerase.\u003c\/p\u003e\n\u003cp\u003eAmpFi 2x qPCR MasterMix has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with AmpFi 2x qPCR MasterMix can be used with TA cloning vectors. The MasterMix contains dye comparable to SYBR Green™ and EvaGreen™.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable height=\"75\" style=\"width: 392.698px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 205px;\"\u003e\u003cstrong\u003e Product Component\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 170.698px;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 205px;\"\u003eAmpFi 2X qPCR MasterMix\u003c\/td\u003e\n\u003ctd style=\"width: 170.698px;\"\u003e2500 rxn (25 ml)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 205px;\"\u003eROX Reference Dye\u003c\/td\u003e\n\u003ctd style=\"width: 170.698px;\"\u003e240 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/AmpFi_2x_qPCR_MasterMix_reliably_quantifies_low_expression_genes-Dr._Vivek_Vaish_1024x1024.jpg?v=1692294194\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/DM-AmpqMM500_AmpFi_2x_qPCR_MasterMix__DataSheet_120623.pdf?v=1705004036\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eProtocol_MM500\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/DM-AmpqMM2500_AmpFi_2X_qPCR_MasterMix_Datasheet_120623.pdf?v=1705004036\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eProtocol_MM2500\u003c\/a\u003e\u003c\/p\u003e","brand":"DMark","offers":[{"title":"500 rxns","offer_id":40652913934380,"sku":"DM-AmpqMM500","price":347.0,"currency_code":"CAD","in_stock":true},{"title":"2500 rxns","offer_id":40652913967148,"sku":"DM-AmpqMM2500","price":1372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/products\/220907Ampq.png?v=1662583870"},{"product_id":"ampfi-2x-probe-qpcr-mastermix","title":"AmpFi 2x Probe qPCR MasterMix","description":"\u003cp\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAmpFi 2x Probe qPCR MasterMix\u003c\/strong\u003e is intended for TaqMan probe-based real-time qPCR analysis of DNA samples. The MasterMix components (dNTPs, DNA polymerase, MgCl2, and other proprietary buffer components) have been created perform well in under one hour. The MasterMix is fit for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. ROX reference dye is provided individually, making it universally compatible with most qPCR instruments.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/AmpFI_480x480.png?v=1669666462\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/DM-AmpPro500_AmpFi_2x_Probe_qPCR_MasterMix__Datasheet_120623.pdf?v=1705004036\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eDataSheet\u003c\/a\u003e\u003c\/p\u003e","brand":"DMark","offers":[{"title":"500 rxns","offer_id":40838026362924,"sku":"DM-AmpPro500","price":347.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/products\/AMPLength1.png?v=1669667008"},{"product_id":"umrt-template-switching-kit","title":"uMRT Template Switching Kit","description":"\u003cp\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eUltraMarathonRT\u003csup\u003eTM\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003eTemplate Switching Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis ideal for library prep for RNA-seq applications. It has consistent and efficient terminal transferase activity, allowing it to be used in applications where template switching is preferred. uMRT can create cDNA from any RNA: long or short RNAs, highly structured or difficult transcripts of any abundance. uMRT used with our Template Switching Oligo (TSO) will allow you to create full-length cDNAs while adding appropriate sequencing adapters. Use the uMRT Template Switching Kit to reduce cDNA library bias.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUsed for bulk sequencing library preparation and low abundant RNA samples in long-read sequencing, SMART-seq, and RACE applications (use with RNA input 0.1 ng – 20 ng).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eUltraMarathonRT Template Switching Kit Components:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eUltraMarathonRT\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e2x RT Buffer\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e5x Template Switching Buffer\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003euMRT-TSO\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLow Boost\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eNuclease-free water\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eCatalog# R1004S (20 reactions), R1004M (50 reactions), R1004L (100 reactions)\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReagents required but not supplied:\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003edNTP mix stock (10 mM each)\n\u003cul\u003e\n\u003cli\u003eThe dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. High purity nucleotides (\u0026gt;99%) are recommended, and the pH should be adjusted to 7.0 with sodium hydroxide.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003edATP stock (10 mM)\n\u003cul\u003e\n\u003cli\u003edATP is needed for the non-templated addition of three adenosines (AAA) to the 3'-end of cDNA primer extension products that facilitates the template switching process.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eCustom oligonucleotides\n\u003cul\u003e\n\u003cli\u003euMRT dT18 primer\n\u003cul\u003e\n\u003cli\u003eCustom oligo-dT primer needed for reverse transcription.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eCustom primer used for full-length cDNA preamplification. (Custom primer sequences are provided in the template switching protocol)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cul\u003e\n\u003cli\u003eAmpPCR primer\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eKAPA HiFi DNA polymerase (Roche, Cat# KK2102) is the recommended DNA polymerase as it provides the most full-length cDNA products\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003eStorage conditions:\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e For short duration of storage (\u0026lt; 3 month), UltraMarathonRT can be placed in a -20℃ freezer. -80℃ is recommended for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.\u003c\/p\u003e\n\u003cdiv id=\"shopify-section-template--25874915918106__custom_banner_mnRCKT\" class=\"shopify-section\"\u003e\n\u003cdiv class=\"full-width custom-banner\"\u003e\n\u003cdiv class=\"banner-inner\"\u003e\n\u003cdiv class=\"banner-text-outer\"\u003e\n\u003cdiv class=\"page-width banner-text\"\u003e\n\u003cdiv class=\"custom-txt\"\u003e\n\u003ch1\u003eUltraMarathonRT\u003csup\u003eR\u003c\/sup\u003e\n\u003c\/h1\u003e\n\u003cp\u003eFinally, a reverse transcriptase that uncovers hidden data, enabling the unbiased exploration of biological complexity.\u003c\/p\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"shopify-section-template--25874915918106__bg_image_with_text_ultra_CG6PVk\" class=\"shopify-section\"\u003e\n\u003cdiv class=\"dynamic-section\"\u003e\n\u003cdiv class=\"content page-width\"\u003e\n\u003ch2\u003eHow much of the transcriptome are you missing?\u003c\/h2\u003e\n\u003cp\u003eThe process of reverse transcription (RT) enables researchers to “see” the transcriptome. However, most RT enzymes lack robust processivity - the ability to efficiently and continuously synthesize cDNA without dissociating from the RNA strand - making the transcription of long, highly structured, or modified RNA transcripts difficult. Furthermore, most RT enzymes operate at temperatures that can damage RNA, jeopardizing the detection of complex and low-abundance transcripts. As a result, researchers may be missing RNA transcripts in their samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"shopify-section-template--25874915918106__ultra_multi_column_yc4cGX\" class=\"shopify-section\"\u003e\n\u003cdiv class=\"feature-grid-section\"\u003e\n\u003cdiv class=\"page-width\"\u003e\n\u003ch2\u003eGenerate unbiased data with an RT enzyme that goes the extra mile\u003c\/h2\u003e\n\u003cp class=\"title-p\"\u003eUltraMarathonRT (uMRT) is a next-generation reverse transcriptase built to reveal transcriptomic diversity in biological samples. Structurally distinct from other RT enzymes, uMRT operates at ambient temperature, preserving RNA integrity. uMRT uses its unmatched helicase activity to unwind complex RNA structures and its enhanced processivity to fully transcribe long, structurally complex transcripts in a single pass and at single-cell levels, enabling you to capture transcript data that is more representative of the diversity in your sample.\u003c\/p\u003e\n\u003cdiv class=\"features-grid\"\u003e\n\u003cdiv class=\"feature-item\"\u003e\n\u003cdiv class=\"feature-inner\"\u003e\n\u003cdiv class=\"feature-front\"\u003e\n\u003cimg class=\"feature-icon\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/icn-info-01_200x200.svg?v=1741266818\" alt=\"Feature Icon\"\u003e\n\u003cp class=\"feature-title\"\u003e\u003cstrong\u003eTranscribe any RNA in a single pass\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-back\"\u003e\n\u003cdiv class=\"feature-description\"\u003e\n\u003cp\u003eTranscribe entire \u0026gt;30kb viral genomes with enhanced enzyme processivity.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-item\"\u003e\n\u003cdiv class=\"feature-inner\"\u003e\n\u003cdiv class=\"feature-front\"\u003e\n\u003cimg class=\"feature-icon\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/icn-info-02_200x200.svg?v=1741265753\" alt=\"Feature Icon\"\u003e\n\u003cp class=\"feature-title\"\u003e\u003cstrong\u003eOperate at ambient temperatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-back\"\u003e\n\u003cdiv class=\"feature-description\"\u003e\n\u003cp\u003ePreserve RNA integrity with lower reaction temperatures 30\u003csup\u003e°\u003c\/sup\u003eC\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-item\"\u003e\n\u003cdiv class=\"feature-inner\"\u003e\n\u003cdiv class=\"feature-front\"\u003e\n\u003cimg class=\"feature-icon\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/icn-info-03_200x200.svg?v=1741266984\" alt=\"Feature Icon\"\u003e\n\u003cp class=\"feature-title\"\u003e\u003cstrong\u003ePower through complex transcripts\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-back\"\u003e\n\u003cdiv class=\"feature-description\"\u003e\n\u003cp\u003eUnmatched helicase activity unwinds complex RNA structure to enable complete 5’ end to 3’ end sequencing of previously invisible regions:\u003cbr\u003e• Highly structured RNAs\u003cbr\u003e• Centromeric regions\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-item\"\u003e\n\u003cdiv class=\"feature-inner\"\u003e\n\u003cdiv class=\"feature-front\"\u003e\n\u003cimg class=\"feature-icon\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/icn-info-04_200x200.svg?v=1741267008\" alt=\"Feature Icon\"\u003e\n\u003cp class=\"feature-title\"\u003e\u003cstrong\u003eDetect RNA modifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-back\"\u003e\n\u003cdiv class=\"feature-description\"\u003e\n\u003cp\u003eUncover RNA modifications:\u003cbr\u003e\u003cbr\u003e• Epitranscriptome\u003cbr\u003e• RNA secondary structures\u003cbr\u003e• RNA editing sites\u003cbr\u003e• Post-transcriptional\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-item\"\u003e\n\u003cdiv class=\"feature-inner\"\u003e\n\u003cdiv class=\"feature-front\"\u003e\n\u003cimg class=\"feature-icon\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/icn-info-05_200x200.svg?v=1741267031\" alt=\"Feature Icon\"\u003e\n\u003cp class=\"feature-title\"\u003e\u003cstrong\u003eSimplify library prep with efficient template switching\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-back\"\u003e\n\u003cdiv class=\"feature-description\"\u003e\n\u003cp\u003eCreate full-length cDNAs in a simplified reaction even when the 5' end of the RNA sequence is unknown.\u003c\/p\u003e\n\u003cdiv class=\"title-of-tab\"\u003e\n\u003cp\u003e\u003cstrong\u003eIdentify more genes\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"content-of-tab\"\u003e\n\u003cdiv class=\"content-of-tab-heading\"\u003e\n\u003cp\u003e\u003cstrong\u003eChanging the standard RT to uMRT enabled the identification of 25% more genes.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"content-of-tab-text\"\u003e\n\u003cp\u003eComparison of uMRT and SSII in a representative SMART-seq experiment.\u003c\/p\u003e\n\u003cp\u003euMRT identified more genes in each RNA class.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-of-tab\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/img-tab-01.webp?v=1741599648\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"image-of-tab\"\u003e\n\u003ch5\u003eGuo L-T., Grinko A., Olson S.,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eet al.\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e(2024). Characterization and implementation of the MarathonRT template-switching reaction to expand the capabilities of RNA-Seq.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eRNA\u003c\/em\u003e. doi:\u003ca href=\"https:\/\/rnajournal.cshlp.org\/content\/30\/11\/1495\" title=\"https:\/\/rnajournal.cshlp.org\/content\/30\/11\/1495\" target=\"_blank\"\u003e10.1261\/rna.080032.124\u003c\/a\u003e.\u003c\/h5\u003e\n\u003cdiv class=\"title-of-tab\"\u003e\n\u003cp\u003e\u003cstrong\u003eDiscover challenging transcripts\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"content-of-tab\"\u003e\n\u003cdiv class=\"content-of-tab-heading\"\u003e\n\u003cp\u003euMRT enables more accurate, unbiased transcriptome analysis\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"content-of-tab-text\"\u003e\n\u003cp\u003eIn a representative experiment, there were no sequencing reads from the SSII dataset mapped to the intron sequence between exon 10 and 11 of the WASH7P gene [top] which contains a stable structure [bottom].\u003c\/p\u003e\n\u003cp\u003eIn contrast, the intron can be efficiently detected by uMRT with its natural helicase activity.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-of-tab\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/img-tab-02.webp?v=1741599669\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"image-of-tab\"\u003e\n\u003cdiv class=\"title-of-tab\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-of-tab\"\u003e\n\u003cdiv class=\"title-of-tab\"\u003e\n\u003cp\u003e\u003cstrong\u003eProtect RNA integrity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"content-of-tab\"\u003e\n\u003cdiv class=\"content-of-tab-heading\"\u003e\n\u003cp\u003euMRT works optimally at ambient temperatures, preserving the integrity of RNA samples\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"content-of-tab-text\"\u003e\n\u003cp\u003eRNA samples (particularly long, structured RNAs or very low abundant RNAs) are delicate and degrade rapidly when heated. uMRT works optimally at 20-42ºC.\u003c\/p\u003e\n\u003cp\u003eIn the figure [right], an HCV RNA was incubated in a diversity of buffer conditions without enzyme to evaluate the impact of temperature on template stability. Samples were analyzed using the Agilent 2100 Bioanalyzer system. Incubation in uMRT buffer at 30ºC shows no degradation of HCV RNA through 30 minutes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-of-tab\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/heat_degradation_3.9.26_1.jpg?v=1773086203\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eDownloads:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/5x_Template_Switching_Buffer_V1.2.pdf?v=1782490032\" target=\"_blank\" rel=\"noopener\"\u003e\u003cspan\u003e5x TS Buffer\u003c\/span\u003e SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Template_Switching_Oligo_SDS_7.1.24_2_1.pdf?v=1782490032\" target=\"_blank\" rel=\"noopener\"\u003euMRT Template Switching Oligo SDS\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/2x_RT_Reaction_Buffer_SDS_V1.1.pdf?v=1782486367\" target=\"_blank\"\u003e2X RT Buffer SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Ultra_MarathonRT_Enzyme_SDS___1.1_1.pdf?v=1782486367\" target=\"_blank\"\u003eUltraMarathonRT SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_Boost_SDS_1.1_1.pdf?v=1782486966\" target=\"_blank\"\u003eHigh Boost SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/cDNA_synthesis_for_in_vivo_SHAPE-MaP_protocol_May2024.pdf?v=1782486367\" target=\"_blank\"\u003eSHAPE-Map Protocol\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/UltraMarathonRT_bulk_RNA_library_prep_protocol_9May2024_1.pdf?v=1782490033\" target=\"_blank\" rel=\"noopener\"\u003eRNA Library Prep Protocol\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_QuickStart_TemSwiKit_051724_1.pdf?v=1782490032\" target=\"_blank\" rel=\"noopener\"\u003eQuick Start Guide\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/26.3.6_CoA_R1004S_24240_TS20_Kits.pdf?v=1782490031\" target=\"_blank\" rel=\"noopener\"\u003eCoA\u003c\/a\u003e\u003c\/p\u003e","brand":"RNA Connect","offers":[{"title":"20 rxns","offer_id":45503287230508,"sku":"RNC-R1004S","price":739.0,"currency_code":"CAD","in_stock":true},{"title":"50 rxns","offer_id":45503287263276,"sku":"RNC-R1004M","price":1662.0,"currency_code":"CAD","in_stock":true},{"title":"100 rxns","offer_id":45503287296044,"sku":"RNC-R1004L","price":2990.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_TSKit_Microtubes_1900x1080_ed5245df-447b-419f-9816-f5b4e92cff8a.jpg?v=1777656009"},{"product_id":"umrt-two-step-rt-pcr-kit","title":"uMRT Two-Step RT-PCR Kit","description":"\u003cp\u003e\u003cspan\u003eThe \u003cstrong\u003eUltraMarathonRT\u003csup\u003e® \u003c\/sup\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cstrong\u003eTwo-Step RT-PCR Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a next-generation solution for researchers needing sensitive and faithful RNA-to-cDNA conversion followed by amplification in RT-PCR experiments, regardless of RNA template length, structure, or GC content.\u003c\/p\u003e\n\u003cp\u003eThe kit is designed for experiments requiring high sensitivity and precision, significantly enhancing gene detection with lengths ranging from hundreds of nucleotides to tens of thousands of nucleotides. Unlike other RT-PCR kits, the UltraMarathonRT RT-PCR Kit requires only 0.1 pg of RNA input for the amplification of RNAs \u0026gt; 10 kb. \u003c\/p\u003e\n\u003cp\u003eUltraMarathonRT is an ultra-processive reverse transcriptase enzyme with intrinsic helicase activity that actively unwinds RNA secondary and tertiary structures naturally without heat, faithfully producing full-length cDNAs from complex, long, or structured RNA templates that standard reverse transcriptases often miss. The DNA polymerase provided in this kit has high fidelity and is extremely processive in amplifying long cDNA (up to 30 kb) generated by UltraMarathonRT.\u003c\/p\u003e\n\u003cp\u003eThis kit is perfect for researchers looking to achieve high-quality results using RT-PCR working with long, complex RNA and includes all necessary reagents.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Applications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression analysis\u003c\/li\u003e\n\u003cli\u003eFull-length isoform analysis\u003c\/li\u003e\n\u003cli\u003eFull-length virus genome analysis\u003c\/li\u003e\n\u003cli\u003eMolecular cloning\u003c\/li\u003e\n\u003cli\u003eLong or structured RNA analysis with low input\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eKit Components:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eUltraMarathonRT\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e2x RT Buffer\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHigh Boost\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eNuclease-free Water\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003edNTP mix (10 mM each)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e2x PCR Master Mix\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRandom primers (15mer) (10 µM)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eOligo(dT)\u003csub\u003e18\u003c\/sub\u003e Primer (5 μM)\u003cbr\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eCatalog# R1005S (20 rxns), R1005M (50 rxns), R1005L (250 rxns) \u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReagents required but not supplied:\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eRNA template: 0.1 pg-2 μg total cellular RNA or 0.1 pg-500 ng of mRNA are recommended for each reaction. If target template is \u0026gt; 10 kb, a minimum input of 1 pg is recommended for both total cellular RNA or mRNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage conditions:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eFor short duration of storage (\u0026lt; 3 month), UltraMarathonRT can be placed in a -20\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e freezer. -80\u003c\/span\u003e\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e is recommended for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv id=\"shopify-section-template--25874915918106__custom_banner_mnRCKT\" class=\"shopify-section\"\u003e\n\u003cdiv class=\"full-width custom-banner\"\u003e\n\u003cdiv class=\"banner-inner\"\u003e\n\u003cdiv class=\"banner-text-outer\"\u003e\n\u003cdiv class=\"page-width banner-text\"\u003e\n\u003cdiv class=\"custom-txt\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"shopify-section-template--25874915918106__ultra_multi_column_yc4cGX\" class=\"shopify-section\"\u003e\n\u003cdiv class=\"feature-grid-section\"\u003e\n\u003cdiv class=\"page-width\"\u003e\n\u003cdiv class=\"features-grid\"\u003e\n\u003cdiv class=\"feature-item\"\u003e\n\u003cdiv class=\"feature-inner\"\u003e\n\u003cdiv class=\"feature-back\"\u003e\n\u003cdiv class=\"feature-description\"\u003e\n\u003cdiv class=\"image-of-tab\"\u003e\n\u003cdiv class=\"image-of-tab\"\u003e\n\u003cdiv class=\"content-of-tab\"\u003e\n\u003cdiv class=\"content-of-tab-text\"\u003e\n\u003csection id=\"shopify-section-template--25874915098906__rich_text_dacUpr\" class=\"shopify-section section\"\u003e\n\u003cdiv class=\"isolate\"\u003e\n\u003cdiv class=\"rich-text content-container color-scheme-1 gradient rich-text--full-width content-container--full-width section-template--25874915098906__rich_text_dacUpr-padding section-template--25874915098906__rich_text_dacUpr\"\u003e\n\u003cdiv class=\"rich-text__wrapper rich-text__wrapper--center page-width\"\u003e\n\u003cdiv class=\"rich-text__blocks center\"\u003e\n\u003ch2 class=\"rich-text__heading rte inline-richtext h2 scroll-trigger animate--slide-in\" data-cascade=\"\"\u003e\u003cstrong\u003eA High-Performance Solution for Sensitive and Precise RNA Analysis\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv class=\"rich-text__text rte scroll-trigger animate--slide-in\" data-cascade=\"\"\u003e\n\u003cp\u003eThe UltraMarathonRT® Two-Step RT-PCR Kit is a next-generation tool designed for researchers seeking unmatched sensitivity, high-fidelity, and efficiency when analyzing RNA. Whether you are studying complex, long, or structured RNA targets, these kits deliver high-fidelity results with exceptional performance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cstrong\u003eConvert and Amplify with Precision\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"rich-text__blocks center\"\u003eThe UltraMarathonRT® Two-Step RT-PCR Kit offers faithful RNA-to-cDNA conversion followed by robust amplification, regardless of the RNA template’s length, secondary structure, or GC content.\u003c\/div\u003e\n\u003cdiv class=\"rich-text__blocks center\"\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003ch4 class=\"rich-text__blocks center\"\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/h4\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv id=\"shopify-section-template--25874915098906__new_key_features_section_tPw9Qx\" class=\"shopify-section\"\u003e\n\u003csection class=\"key-features-section page-width\"\u003e\n\u003cdiv class=\"row green\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eUnparalleled Sensitivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eRequires as little as\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e0.1 pg of input RNA\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eto amplify RNAs \u0026gt;10 kb, enabling detection of even low-abundance targets.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/HeLa_Total_RNA_input.png?v=1738086415\" width=\"600\" height=\"600\" alt=\"\"\u003e\n\u003cp\u003eSensitivity of uMRT two-step RT-PCR for RNA input as low as 0.1 pg\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row blue\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eProcessive Enzyme\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePowered by UltraMarathonRT®, an ultra-processive reverse transcriptase with\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eintrinsic helicase activity\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethat actively unwinds RNA secondary and tertiary structures without heat, producing\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003efull-length cDNA\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ewith exceptional fidelity.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row green\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eLong-Read Capability:\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eEfficiently amplifies cDNA products\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eup to 30 kb\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eusing a highly processive, high-fidelity DNA polymerase.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/ee1c592c1442529e38707fdcf0e6445b.png?v=1738075399\" width=\"600\" height=\"600\" alt=\"\"\u003e\n\u003cp\u003eAmplification of transcripts from 1.2 kb to 14.9 kb using 100 ng Hela total RNA. The cDNA’s generated by Induro RT were amplified by LongAmp Taq. Reverse transcription was performed using Oligo(dT) 18 primer.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row blue\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eVersatile Applications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIdeal for experiments requiring accurate gene detection, from short transcripts to tens of thousands of nucleotides in the areas of gene expression analysis, full-length isoform analysis, full-length virus genome analysis, molecular cloning, long or structured RNA analysis with low input.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eUltraMarathonRT Two-Step RT-PCR Kit\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePerfect for researchers working with long, complex RNA templates who demand high-quality RT-PCR results with maximum sensitivity and precision.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eDownloads:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/UltraMarathonRT_Brochure_RT-PCR.pdf?v=1782496720\" target=\"_blank\"\u003eBrochure\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/2x_RT_Reaction_Buffer_SDS_V1.1.pdf?v=1782486367\" target=\"_blank\"\u003e2X RT Buffer SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Ultra_MarathonRT_Enzyme_SDS___1.1_1.pdf?v=1782486367\" target=\"_blank\"\u003eUltraMarathonRT SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_Boost_SDS_1.1_1.pdf?v=1782486966\" target=\"_blank\"\u003eHigh Boost SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/dNTP_Mix_SDS_V1.1.pdf?v=1782496720\" target=\"_blank\"\u003e\u003cspan\u003edNTP mix (10 mM each) SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca style=\"font-size: 0.875rem;\" rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Random_Primer_15mer__SDS_V1.0.pdf?v=1782496720\" target=\"_blank\"\u003eRandom primers (15mer) (10 µM) SDS\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca style=\"font-size: 0.875rem;\" rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Oligo_dT_18_Primer_SDS_V1.0.pdf?v=1782496720\" target=\"_blank\"\u003eOligo(dT)18 Primer (5 μM)\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca style=\"font-size: 0.875rem;\" rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/UltraMarathonRT_Two-Step_RT-PCR_Kit_Protocol_4.24.25.pdf?v=1782496720\" target=\"_blank\"\u003euMRT Two-Step RT-PCR Kit Protocol\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca style=\"font-size: 0.875rem;\" rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_QuickStart_Two_Step_RT_PCR_Kit.pdf?v=1782496720\" target=\"_blank\"\u003eRT-PCR Quick Start Guide\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"RNA Connect","offers":[{"title":"20 rxns","offer_id":45503333728300,"sku":"RNC-R1005S","price":414.0,"currency_code":"CAD","in_stock":true},{"title":"50 rxns","offer_id":45503333761068,"sku":"RNC-R1005M","price":941.0,"currency_code":"CAD","in_stock":true},{"title":"250 rxns","offer_id":45503333793836,"sku":"RNC-R1005L","price":4132.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/RNAConnect_RTPCRproductrelease_PRimage.png?v=1777656167"},{"product_id":"umrt-two-step-rt-qpcr-kit","title":"uMRT Two-Step RT-qPCR Kit","description":"\u003cp class=\"MsoNormal\"\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cb\u003eUltraMarathonRT® Two-Step RT-qPCR Kit\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003eprovides the most sensitive and reliable quantitation of gene expression on the market. Since the UltraMarathonRT enzyme is unphased by GC stretches and secondary structures of RNA templates, this RT-qPCR kit provides consistent C\u003csub\u003et\u003c\/sub\u003e\u003cspan\u003e \u003c\/span\u003evalues regardless of primer location ensuring accurate, reliable results each time.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eKey Features and Advantages:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003eData\u003cspan\u003e \u003c\/span\u003e\u003ca\u003eReliability\u003c\/a\u003e\u003c\/b\u003e\u003cspan\u003e: Provides consistent C\u003csub\u003et\u003c\/sub\u003e value regardless of primer type (e.g. gene specific, oligo(dT) or randomer) or location. Other RT-qPCR kits will provide widely different C\u003csub\u003et\u003c\/sub\u003e values if primers are in high vs low GC content areas, whereas the \u003cb\u003eUltraMarathonRT® Two-Step RT-qPCR Kit \u003c\/b\u003ewill provide consistent C\u003csub\u003et\u003c\/sub\u003e values regardless of location.\u003c\/span\u003e\u003cspan class=\"MsoCommentReference\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003ca\u003e\u003cb\u003eRobust Performance\u003c\/b\u003e: Achieve exceptional sensitivity and efficiency, ideal for detecting low-abundance RNA, viral RNA, non-coding RNAs, long RNAs, and even highly structured RNAs. This makes it a versatile tool for a wide range of molecular biology applications.\u003c\/a\u003e\u003cspan class=\"MsoCommentReference\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003ca\u003e\u003cspan\u003e\u003cb\u003eHigh Inhibitor Tolerance\u003c\/b\u003e\u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003e\u003cspan class=\"MsoCommentReference\"\u003e\u003c\/span\u003e\u003c\/span\u003e: Consistent C\u003csub\u003et\u003c\/sub\u003e\u003cspan\u003e \u003c\/span\u003evalues are maintained in the presence of inhibitory compounds potentially found in biological sample preparation such as ethanol (resistant to 10%), isopropanol (resistant to 5%), TRIzol (resistant to 0.7%), Formalin (resistant to 0.0025%), Ammonium Acetate (resistant to 50mM), Bile Salts (resist to 0.05%), and Hematin (resistant to 10 µM).\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003eBroad Dynamic Range\u003c\/b\u003e: Capable of detecting RNA across a wide spectrum of input concentrations ranging from 0.1 pg to 2 µg, providing unparalleled flexibility in experimental design.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan\u003e\u003cb\u003eMinimal Experimental Time:\u003c\/b\u003e\u003c\/span\u003e\u003cspan\u003e An optimized protocol allows researchers to generate complete RT-qPCR data in under 1 hour.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003ePreserves RNA Template Integrity\u003c\/b\u003e: UltraMarathonRT has intrinsic helicase activity for RNA structure unwinding, making the high temperature reaction conditions utilized by other RTs totally unnecessary.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003eDye-Based Detection\u003c\/b\u003e: Incorporates reliable dye-based technology to facilitate accurate and reproducible real-time monitoring of RNA levels.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cb\u003eIdeal Companion for uMRT RNA-seq Validation:\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003eOnly the\u003cspan\u003e \u003c\/span\u003e\u003cb\u003eUltraMarathonRT® Two-Step RT-qPCR Kit\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003ecan reliably validate novel genes detected in uMRT RNA-seq data sets (genes that are undetected by other RTs).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003eThis product is a powerful tool for researchers conducting gene expression analysis and studies involving non-coding and long RNAs.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eKey Applications:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\"\u003eRNA-Seq verification and quantification\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan class=\"MsoCommentReference\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/span\u003eQuantification of small non-coding RNAs (e.g., miRNAs)\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eMutation Detection and Allele Discrimination\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eQuantification of Alternative splice variants\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eMeasurement of RNA degradation\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eQuantification of RNA editing\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eVerification of RNAi knockdown\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003emiRNA Target validation\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eEvaluation of CRISPR experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003eIts companion products, including uMRT kits for cDNA library preparation for RNA-Seq, further enhance its versatility, making it an ideal choice for comprehensive RNA studies.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eWith its high performance, ease of use, and robust capabilities, this kit is tailored to meet the demands of modern molecular biology research, delivering precise and reliable results every time.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eKit Components:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\"\u003eUltraMarathonRT\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003ca\u003e2x RT Buffer Q\u003c\/a\u003e\u003cspan class=\"MsoCommentReference\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eNuclease-free Water\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003edNTP Mix (10 mM each)\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eRandom Primers (15mer) (10 µM)\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eOligo(dT)\u003cspan\u003e\u003csub\u003e18\u003c\/sub\u003e\u003c\/span\u003e\u003cspan\u003e Primer (5 μM)\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e2x qPCR Green Dye Master Mix\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003eCatalog# R1006S (200 rxns), R1006M (500 rxns), R1006L (1,000 rxns) \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eReagents required but not supplied: \u003cspan class=\"msoIns\"\u003e\u003cins datetime=\"2024-12-20T11:07\" cite=\"mailto:George%20Maio\"\u003e\u003c\/ins\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRNA template: 0.1 pg - 2 μg total cellular RNA or 0.1 pg - 500 ng of mRNA are recommended for each reaction.\u003c\/li\u003e\n\u003cli\u003ePCR Primers: Because uMRT provides full 5’-3’ RNA transcript coverage, PCR amplicon location along uMRT’s full-length cDNA molecule synthesized using gene-specific primer or oligo(dT) primer does not affect qPCR quantification. This greatly reduces the potential for bias and\/or C\u003cspan\u003e\u003csub\u003et\u003c\/sub\u003e\u003c\/span\u003e\u003cspan\u003e value variability observed when using randomers with non-processive retroviral RTs.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003eGene-Specific RT primers.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eStorage conditions: \u003c\/b\u003eFor short duration of storage (\u0026lt; 3 month), UltraMarathonRT can be stored in a -20\u003cspan\u003e℃\u003c\/span\u003e freezer. -80\u003cspan\u003e℃\u003c\/span\u003e is recommended for long-term storage. All other kit components are stable in a -20\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003efreezer for long-term storage. Note that UltraMarathonRT and the 2x qPCR Green Dye Master Mix can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.\u003c\/p\u003e\n\u003csection id=\"shopify-section-template--25874915295514__rich_text_dacUpr\" class=\"shopify-section section\"\u003e\n\u003cdiv class=\"isolate\"\u003e\n\u003cdiv class=\"rich-text content-container color-scheme-1 gradient rich-text--full-width content-container--full-width section-template--25874915295514__rich_text_dacUpr-padding section-template--25874915295514__rich_text_dacUpr\"\u003e\n\u003cdiv class=\"rich-text__wrapper rich-text__wrapper--center page-width\"\u003e\n\u003cdiv class=\"rich-text__blocks center\"\u003e\n\u003ch2 class=\"rich-text__heading rte inline-richtext h2 scroll-trigger animate--slide-in\" data-cascade=\"\"\u003e\n\u003cstrong\u003eUnmatched Sensitivity: Enhancing and Streamlining Gene Expression Analysis\u003c\/strong\u003e \u003c\/h2\u003e\n\u003cdiv class=\"rich-text__text rte scroll-trigger animate--slide-in\" data-cascade=\"\"\u003e\n\u003cp\u003eThe \u003cstrong\u003eUltraMarathonRT® Two-Step RT-qPCR Kit\u003c\/strong\u003e  provides the most sensitive and reliable quantitation of gene expression on the market. Since the UltraMarathonRT enzyme is unphased by GC stretches and secondary structures of RNA templates, this RT-qPCR kit provides consistent C\u003csub\u003et\u003c\/sub\u003e values regardless of primer location, ensuring accurate, reliable results each time.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"rich-text__buttons scroll-trigger animate--slide-in\" data-cascade=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/White_Paper_Improved_RT-qPCR_Data_and_Consistency_Using_UltraMarathonRT_9.18.25.pdf?v=1782498449\" target=\"_blank\" rel=\"noopener\"\u003eRead the White Paper\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv id=\"shopify-section-template--25874915295514__new_image_with_banner_KR8XR9\" class=\"shopify-section section\"\u003e\n\u003cdiv class=\"section-template--25874915295514__new_image_with_banner_KR8XR9-padding gradient color-scheme-1 mlv-image-with-text-main\"\u003e\n\u003cdiv class=\"page-width\"\u003e\n\u003cdiv class=\"image-with-text image-with-text--no-overlap isolate scroll-trigger animate--slide-in\"\u003e\n\u003cdiv class=\"image-with-text__grid grid grid--gapless grid--1-col grid--2-col-tablet\"\u003e\n\u003cdiv class=\"image-with-text__media-item image-with-text__media-item--medium image-with-text__media-item--middle grid__item\"\u003e\n\u003cdiv class=\"enlarge-div\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"image-with-text__text-item grid__item\"\u003e\n\u003cdiv id=\"ImageWithText--template--25874915295514__new_image_with_banner_KR8XR9\" class=\"image-with-text__content image-with-text__content--middle image-with-text__content--desktop-left image-with-text__content--mobile-center image-with-text__content--medium content-container background-transparent\"\u003e\n\u003ch2 class=\"image-with-text__heading inline-richtext h2\"\u003e\n\u003cstrong\u003eReliable, Precise, and Timely\u003c\/strong\u003e \u003c\/h2\u003e\n\u003cdiv class=\"image-with-text__text rte body\"\u003e\n\u003cp\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eUltraMarathonRT® Two-Step RT-qPCR Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eensures unmatched data reliability by providing consistent C\u003csub\u003et\u003c\/sub\u003e\u003cspan\u003e \u003c\/span\u003evalues regardless of primer type, RNA complexity, or GC content, eliminating biases and ensuring accurate quantification. With high inhibitor tolerance and a streamlined protocol that delivers results in under an hour, this versatile kit is ideal for RNA-seq validation, small RNA quantification, alternative splicing analysis, and CRISPR experiment evaluation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"shopify-section-template--25874915295514__new_key_features_section_tPw9Qx\" class=\"shopify-section\"\u003e\n\u003csection class=\"key-features-section page-width\"\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cdiv class=\"row green\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eData Reliability\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eProvides consistent C\u003csub\u003et\u003c\/sub\u003e value regardless of primer type (e.g. gene specific, oligo(dT) or randomer) or location. Other RT-qPCR kits will provide widely different C\u003csub\u003et\u003c\/sub\u003e values if primers are in high vs low GC content areas, whereas the \u003cstrong\u003eUltraMarathonRT® Two-Step RT-qPCR Kit \u003c\/strong\u003ewill provide consistent C\u003csub\u003et\u003c\/sub\u003e values regardless of location.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Data_Reliability_image.png?v=1740034733\" width=\"600\" height=\"600\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cstrong\u003eRobust Performance\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row blue-with-image\"\u003e\n\u003cdiv class=\"column\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Robust_Performance_image.png?v=1740034733\" width=\"600\" height=\"600\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eAchieve exceptional sensitivity and efficiency,\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003eideal for detecting low abundance RNA, viral RNA, non-coding RNAs, long RNAs, and even highly structured RNAs\u003c\/strong\u003e. This makes it a versatile tool for a wide range of molecular biology applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row green\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Inhibitor Tolerance\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent C\u003csub\u003et\u003c\/sub\u003e values are maintained in the presence of inhibitory compounds\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003epotentially found in biological sample preparation such as ethanol (resistant to 10%), isopropanol (resistant to 5%), TRIzol (resistant to 0.7%), Formalin (resistant to 0.0025%), Ammonium Acetate (resistant to 50mM), Bile Salts (resistant to 0.05%), and Hematin (resistant to 10µM).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/High_Inhibitor_Tolerance_image.png?v=1740034733\" width=\"600\" height=\"600\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cstrong\u003eBroad Dynamic Range\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row blue-with-image\"\u003e\n\u003cdiv class=\"column\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Broad_Dynamic_Range_image.png?v=1740034734\" width=\"600\" height=\"600\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eCapable of detecting RNA across a wide spectrum of input concentrations ranging from 0.1 pg to 2 µg, providing unparalleled flexibility in experimental design.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row green\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eMinimal Experimental Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eAn optimized protocol allows researchers to generate complete RT-qPCR data in under 1 hour.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Minimal_Experimental_Time_image.png?v=1740034733\" width=\"600\" height=\"600\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cstrong\u003ePreserves RNA Template Integrity\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row blue-with-image\"\u003e\n\u003cdiv class=\"column\"\u003e\u003cimg src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Preserves_RNA_Template_Integrity_image.png?v=1740034733\" width=\"600\" height=\"600\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eUltraMarathonRT has intrinsic helicase activity for RNA structure unwinding, making the high temperature reaction conditions utilized by other RTs totally unnecessary.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row green-without-image\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eDye-Based Detection\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eIncorporates reliable dye-based technology to facilitate accurate and reproducible real-time monitoring of RNA levels.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row blue\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eIdeal Companion for uMRT RNA-seq Validation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eOnly the \u003cstrong\u003eUltraMarathonRT® Two-Step RT-qPCR Kit\u003c\/strong\u003e can reliably validate novel genes detected in uMRT RNA-seq data sets (genes that are undetected by other RTs).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePerfect for researchers\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eworking with\u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003elow abundance RNA, viral RNA, non-coding RNAs, long RNAs, and even highly structured RNA templates \u003c\/span\u003e\u003cstrong\u003ewho demand enhanced gene expression analysis via RT-qPCR\u003c\/strong\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003e\u003cstrong\u003eDownloads:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/2x_RT_Reaction_Buffer_SDS_V1.1.pdf?v=1782486367\" target=\"_blank\"\u003e2X RT Buffer SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/2x_RT_Reaction_Buffer_Q_SDS_V1.0.pdf?v=1782498448\" target=\"_blank\" rel=\"noopener\"\u003e2X RT Reaction Buffer Q SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Ultra_MarathonRT_Enzyme_SDS___1.1_1.pdf?v=1782486367\" target=\"_blank\"\u003eUltraMarathonRT SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_Boost_SDS_1.1_1.pdf?v=1782486966\" target=\"_blank\"\u003eHigh Boost SDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/dNTP_Mix_SDS_V1.1.pdf?v=1782496720\" target=\"_blank\"\u003e\u003cspan\u003edNTP mix (10 mM each) SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Random_Primer_15mer__SDS_V1.0.pdf?v=1782496720\" target=\"_blank\"\u003e\u003cspan\u003eRandom primers (15mer) (10 µM) SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Oligo_dT_18_Primer_SDS_V1.0.pdf?v=1782496720\" target=\"_blank\"\u003e\u003cspan\u003eOligo(dT)18 Primer (5 μM)\u003c\/span\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/2x_qPCR_Green_Dye_Master_Mix_SDS_V1.0.pdf?v=1782498448\" target=\"_blank\" rel=\"noopener\"\u003e\u003cspan\u003e2x qPCR Green-Dye Master Mix SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/UltraMarathonRT_Two-Step_RT-qPCR_Kit_Protocol_4.28.25.pdf?v=1782498448\" target=\"_blank\" rel=\"noopener\"\u003e\u003cspan\u003euMRT Two-Step RT-qPCR Kit Protocol\u003c\/span\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"s_tab\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_QuickStart_Two-Step_RT-qPCR_Kit_1.7.24._FINAL.pdf.pdf?v=1782498449\" target=\"_blank\" rel=\"noopener\"\u003e\u003cspan\u003eRT-qPCR Quick Start Guide\u003c\/span\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e","brand":"RNA Connect","offers":[{"title":"200 rxns","offer_id":45503350308908,"sku":"RNC-R1006S","price":695.0,"currency_code":"CAD","in_stock":true},{"title":"500 rxns","offer_id":45503350341676,"sku":"RNC-R1006M","price":1706.0,"currency_code":"CAD","in_stock":true},{"title":"1000 rxns","offer_id":45503350374444,"sku":"RNC-R1006L","price":3350.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/RT-qPCR_Kit_Microtubes_1900x1080_websiteedit.png?v=1777656325"},{"product_id":"umrt-cdna-synthesis-amplification-kit","title":"uMRT cDNA Synthesis \u0026 Amplification Kit","description":"\u003cp\u003e\u003cspan\u003eThe \u003cstrong\u003eUltraMarathonRT® (uMRT) cDNA Synthesis \u0026amp; Amplification Kit\u003c\/strong\u003e enables you to see more of the transcriptome in each sample. uMRT is a next-generation reverse transcriptase that synthesizes end-to-end cDNA in a single pass from even the most complex RNA molecules—long, structured, high-GC, or low-abundance.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eConventional retroviral RTs are ‘distributive’ meaning they attach and detach many times from each RNA transcript which often creates truncated, incomplete, or incorrect cDNAs. This is often caused by secondary structures or repeat sequences within the template.\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUltraMarathonRT solves this problem. It is a highly engineered group II intron-encoded reverse transcriptase with intrinsic helicase activity. It bypasses the drop-offs, biases, and blind spots common in traditional cDNA synthesis workflows.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eWhy Choose This Kit\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eHigher Gene Detection \u0026amp; Isoform Sensitivity\u003c\/strong\u003e\u003cbr\u003eCaptures more genes and isoforms, including alternative splicing and intron retention events that other kits miss.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003eSuperior Amplification Accuracy\u003c\/strong\u003e\u003cbr\u003eUniform gene body coverage improves the accuracy of differential gene expression analysis.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cstrong\u003eDesigned for Modern RNA-Seq Applications\u003c\/strong\u003e\u003cbr\u003ePerfect for short-read sequencing, biomarker discovery, and therapeutic target validation.\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eBulk RNA-Seq\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eShort-read RNA-Seq\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLow-abundance transcript detection\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eIsoform-level resolution\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAlternative splicing analysis\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eRNA biomarker discovery\u003cbr\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eUltraMarathonRT cDNA Synthesis \u0026amp; Amplification Kit Components:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv data-olk-copy-source=\"MailCompose\"\u003e\n\u003cul\u003e\n\u003cli\u003eUltraMarathonRT (20 U\/µL)\u003c\/li\u003e\n\u003cli\u003e2x RT Buffer S\u003c\/li\u003e\n\u003cli\u003edNTP mix (10 mM each)\u003c\/li\u003e\n\u003cli\u003edATP solution (10 mM)\u003c\/li\u003e\n\u003cli\u003euMRT oligo(dT) primer (5 μM)\u003c\/li\u003e\n\u003cli\u003euMRT-TSO_v2\u003c\/li\u003e\n\u003cli\u003eAmpPCR\u003c\/li\u003e\n\u003cli\u003e5x Template Switching Buffer\u003c\/li\u003e\n\u003cli\u003eNuclease-free water\u003c\/li\u003e\n\u003cli\u003e5x DNA Polymerase Buffer\u003c\/li\u003e\n\u003cli\u003eDNA Polymerase (1 U\/µL)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"extra-thins\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca\u003e\u003cb\u003eReagents Required but Not Supplied:\u003c\/b\u003e\u003c\/a\u003e\u003cspan class=\"MsoCommentReference\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\"\u003eRNA template: 1 ng – 100 ng total cellular RNA or 0.1 ng – 20 ng mRNA is recommended for each reaction.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003ePost PCR clean-up reagent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog#\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eR1007S (20 reactions), R1007M (50 reactions), R1007L (100 reactions)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eFor short duration of storage (\u0026lt; 6 month), UltraMarathonRT can be stored in a -20℃ freezer. -80℃ is recommended for long-term storage. All other kit components are stable in a -20℃ freezer for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv id=\"shopify-section-template--25874916049178__custom_banner_piAdXd\" class=\"shopify-section\"\u003e\n\u003cdiv class=\"full-width custom-banner\"\u003e\n\u003cdiv class=\"banner-inner\"\u003e\n\u003cdiv class=\"banner-text-outer\"\u003e\n\u003cdiv class=\"page-width banner-text\"\u003e\n\u003cdiv class=\"custom-txt\"\u003e\n\u003ch1\u003eReveal previously undetectable RNAs\u003c\/h1\u003e\n\u003cp\u003eSee the transcriptome with greater depth, clarity and confidence with the UltraMarathonRT\u003csup\u003eR\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecDNA Synthesis \u0026amp; Amplification Kit.\u003c\/p\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/UltraMarathonRT_cDNA_Synthesis_Amplification_White_Paper.pdf?v=1782500951\" target=\"_blank\"\u003eDownload the Whitepaper\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"custom-txt\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"shopify-section-template--25874916049178__custom_bg_image_with_text_bByYqb\" class=\"shopify-section\"\u003e\n\u003cdiv class=\"dynamic-section\"\u003e\n\u003cdiv class=\"content page-width\"\u003e\n\u003ch2\u003e\n\u003cspan\u003eUncover the RNAs that retroviral reverse transcriptases miss.\u003c\/span\u003e\u003cspan\u003eSwitch to the UltraMarathonRT-powered cDNA Synthesis \u0026amp; Amplification Kit\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cdiv class=\"descriptionWrapper\"\u003e\n\u003cp\u003eRNA-seq is a powerful method for studying gene expression, but commonly used retroviral reverse transcriptases (e.g., MMLV and AMV) lack two critical properties for effective reverse transcription: intrinsic helicase activity, which enables the unwinding of complex RNA secondary structures, and high processivity, which allows for the synthesis of full-length cDNA from long RNA molecules.\u003c\/p\u003e\n\u003cp\u003eUltraMarathonRT (uMRT) is a reverse transcriptase enzyme designed to overcome these limitations and improve RNA coverage. The cDNA Synthesis \u0026amp; Amplification Kit, powered by uMRT, combines intrinsic helicase activity with ultraprocessivity to efficiently reverse transcribe long RNA sequences (\u0026gt;30 kb—compared to the ~2 kb typically transcribed by retroviral RTs), as well as RNA with high GC content, complex structures, or low abundance. uMRT also functions at lower temperatures, helping preserve RNA integrity and enabling a more comprehensive view of the transcriptome.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"shopify-section-template--25874916049178__custom_imgleft_textright_fjn3YP\" class=\"shopify-section\"\u003e\n\u003cdiv class=\"ultra-marathon-wrapper-section\"\u003e\n\u003cdiv class=\"content page-width\"\u003e\n\u003ch2 class=\"ultra-marathon-wrapper-section-heading\"\u003ePower through RNA complexity and boost sample coverage\u003c\/h2\u003e\n\u003cdiv class=\"ultra-marathon-section\"\u003e\n\u003cdiv class=\"left-image\"\u003e\u003cimg height=\"358\" width=\"358\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/img-kit_1024x1024.jpg?v=1755761905\" alt=\"UltraMarathonRT Image\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"right-content\"\u003e\n\u003ch2\u003eThe UltraMarathonRT cDNA Synthesis \u0026amp; Amplification Kit enables superior quantification of:\u003c\/h2\u003e\n\u003chr class=\"separator\"\u003e\n\u003cdiv class=\"custom-discription\"\u003e\n\u003cul\u003e\n\u003cli\u003eHighly structured RNAs\u003c\/li\u003e\n\u003cli\u003eLong RNAs\u003c\/li\u003e\n\u003cli\u003eTranscripts with high GC content\u003c\/li\u003e\n\u003cli\u003eNon-coding RNAs\u003c\/li\u003e\n\u003c\/ul\u003e\nGo from RNA to Full-Length cDNA in less than 3 hours with our streamlined workflow and obtain greater insights from your RNA-seq experiments.\u003c\/div\u003e\n\u003ca href=\"https:\/\/rnaconnect.com\/pages\/ultramarathonrt\" class=\"cta-button-custom\"\u003e\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"shopify-section-template--25874916049178__mlv_info_cards_with_popup_jYEjDa\" class=\"shopify-section\"\u003e\n\u003csection class=\"info-cards-section\"\u003e\n\u003cdiv class=\"page-width text-center\"\u003e\n\u003ch2\u003eGreater visibility into the transcriptome\u003c\/h2\u003e\n\u003cdiv class=\"section-content mlcontent\"\u003e\n\u003cp\u003eAchieve a more accurate and comprehensive view of the transcriptome—at the same cost as leading kits. Click the cards below to explore how the cDNA Synthesis \u0026amp; Amplification Kit, powered by uMRT, delivers deeper insights from RNA samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"info-cards-wrapper\"\u003e\n\u003cdiv class=\"info-card\" data-popup=\"card_WWHgic\"\u003e\u003cstrong\u003e10-30% more genes\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"info-card\" data-popup=\"card_WWHgic\"\u003e\u003cimg alt=\"popup image\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Graph_1_10-30_more_genes_1000x.png?v=1758304543\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"info-card\" data-popup=\"card_xAkR7T\"\u003e\u003cstrong\u003eBetter RNA Quantification\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"info-card\" data-popup=\"card_xAkR7T\"\u003e\u003cimg alt=\"popup image\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Graph_2_better_rna_quantification_1000x.png?v=1758304627\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"info-card\" data-popup=\"card_RqeCCN\"\u003e\u003cstrong\u003eLess Bias\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"info-card\" data-popup=\"card_RqeCCN\"\u003e\u003cimg alt=\"popup image\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Graph_3_1000x.png?v=1756135454\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"info-card\" data-popup=\"card_ABnQVh\"\u003e\u003cstrong\u003e2x More Retained Introns\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"info-card\" data-popup=\"card_ABnQVh\"\u003e\u003cimg alt=\"popup image\" src=\"https:\/\/rnaconnect.com\/cdn\/shop\/files\/Graph_4_1000x.png?v=1756135453\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use Only. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDownloads:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/UltraMarathonRT_Sales_One_Pager_cDNA_Synthesis_Amplification_6.10.25_Interest_Form.pdf?v=1782500951\" target=\"_blank\"\u003eBrochure\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/Ultra_MarathonRT_Enzyme_SDS___1.1_1_6242a76d-46d8-41e4-891f-fe0b82705ad1.pdf?v=1782500949\" target=\"_blank\"\u003e\u003cspan\u003eUltraMarathonRT SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/2x_RT_Reaction_Buffer_S_SDS_V1.0.pdf?v=1782500950\" target=\"_blank\"\u003e\u003cspan\u003e2x RT Reaction Buffer S SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/dNTP_Mix_SDS_V1.1_1.pdf?v=1782500949\" target=\"_blank\"\u003e\u003cspan\u003edNTP mix (10 mM each) SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/dATP_Solution_SDS_V1.0.pdf?v=1782500950\"\u003e\u003cspan\u003edATP Solution SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_oligo_dT_primer_SDS_V1.0.pdf?v=1782500950\" target=\"_blank\"\u003e\u003cspan\u003euMRT oligo(dT) primer (5 μM) SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT-TSO_v2_SDS_V1.0.pdf?v=1782500950\"\u003e\u003cspan\u003euMRT-TSO_v2 SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/AmpPCR_SDS_V1.0.pdf?v=1782500950\" target=\"_blank\"\u003e\u003cspan\u003eAmpPCR SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/5x_Template_Switching_Buffer_V1.2_1.pdf?v=1782500949\" target=\"_blank\"\u003e\u003cspan\u003e5x Template Switching Buffer SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/5x_DNA_Polymerase_Buffer_SDS_V1.0.pdf?v=1782500950\" target=\"_blank\"\u003e\u003cspan\u003e5x DNA Polymerase Buffer SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/DNA_Polymerase_SDS_V1.0.pdf?v=1782500950\" target=\"_blank\"\u003e\u003cspan\u003eDNA Polymerase SDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/uMRT_cDNA_Synthesis_Amplification_Kit_for_RNA-Seq_Library_Preparation_7.21.25.pdf?v=1782501347\" target=\"_blank\" rel=\"noopener\"\u003e\u003cspan\u003ecDNA Synthesis \u0026amp; Amplification Protocol\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv class=\"page-width\"\u003e\n\u003cdiv class=\"tabs\"\u003e\n\u003cdiv class=\"custom-tabs\"\u003e\n\u003cdiv class=\"tab-body\"\u003e\n\u003cdiv id=\"tab4\" class=\"inner-tab active-tab\"\u003e\n\u003cdiv class=\"s-tabbb\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/R1007S_24120.pdf?v=1782500949\" target=\"_blank\"\u003e\u003cspan\u003eR1007S_24120 CoA\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/R1007M_24132.pdf?v=1782500949\" target=\"_blank\"\u003e\u003cspan\u003eR1007M_24132 CoA\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/26.3.6_CoA_R1007S_24241_SynAmp20_kits.pdf?v=1782500949\"\u003e\u003cspan\u003eR1007S_24241 CoA\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"color-scheme-1 gradient\"\u003e\n\u003cdiv class=\"section-template--25874918211866__custom_liquid_Ja8jQr-padding\"\u003e\n\u003cdiv class=\"publications\"\u003e\n\u003cdiv class=\"page-width\"\u003e\n\u003cdiv class=\"heading\"\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"RNA Connect","offers":[{"title":"20 rxns","offer_id":45503357452332,"sku":"RNC-R1007S","price":783.0,"currency_code":"CAD","in_stock":true},{"title":"50 rxns","offer_id":45503357485100,"sku":"RNC-R1007M","price":1829.0,"currency_code":"CAD","in_stock":true},{"title":"100 rxns","offer_id":45503357517868,"sku":"RNC-R1007L","price":3429.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/files\/cDNAsynth_Kit_Microtubes_1920x1080_68987085-3d9a-4f47-b058-4bccb34c6414.png?v=1777656627"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0377\/2163\/6908\/collections\/qPCR.jpg?v=1595365765","url":"https:\/\/dmarkbio.com\/collections\/qpcr\/rna-seq.oembed","provider":"DMark","version":"1.0","type":"link"}