TELL-Seq™ WGS Library Reagent Box 1, RUO


Price:
$468

Description

Research Use Only. 

TELL-Seq™ WGS Library Reagent Box 1, RUO is a part of the standard bundle.

The standard bundle has enough reagent to prepare 4 sample libraries for genomes 1 Gb in size or larger or 12 sample libraries for small genomes up to 200 Mb in size for both diploid and haploid genomes.

The standard bundle includes the following:

SureSelect users will also need a UST TELL-Seq™ Target Blocker 8-Rxn Kit

TELL-Seq™ WGS LIBRARY PREP KIT

Features:

  • LONG READ NGS LIBRARY PREPARATION METHOD

  • EASE OF USE: HANDS-ON TIME ( < 1 HOUR)

  • SINGLE TUBE PROCESS: SEQUENCING READY LIBRARY IN 3 HOURS

  • LOW DNA INPUT: 0.5NG TO 5NG DEPENDING ON GENOME SIZE

  • HIGHLY SCALABLE FOR SMALL AND LARGE GENOMES

  • EASILY AUTOMATED

TELL-Seq™ Technology

Simple and scalable genome analysis

UST’s Transposase Enzyme-Linked Long-read Sequencing (UST TELL-Seq™) is a simple and scalable NGS library technology that generates barcode linked-reads for genome-scale sequencing applications. The whole procedure can be carried out in a PCR tube without the need for expensive instrumentation. The TELL-Seq™ Whole Genome Sequencing (WGS) Library Prep Kit will generate an Illumina sequencing library in just 3 hours. The protocol can be easily adjusted based on the genome size to be analyzed. TELL-Seq will be the new standard library method for WGS.



TELL-Seq™ Library Preparation and Sequencing Workflow

TELL-Seq Library Preparation and Sequencing Workflow

TELL-Seq Library Preparation and Sequencing Workflow

 


How it works

How it works.png


Results

RESULTS I: IGV SNAPSHOT OF TELL-SEQ LINKED READS

Results I: IGV Snapshot of TELL-Seq Linked ReadsRESULTS II: TELL-SEQ RESULTS FOR THREE BACTERIA WITH DIFFERENT GC CONTENT

Results II: TELL-Seq Results for Three Bacteria with Different

 

RESULTS III: DE NOVO ASSEMBLY RESULTS COMPARED WITH REFERENCE

Results III: Novo Assembly Results Compared with Reference

 

RESULTS IV: WHOLE GENOME HAPLOTYPE PHASING RESULTS

Results IV: Whole Genome Haplotype Phasing Results

Applications:

de novo assembly icon.PNG

DE NOVO ASSEMBLY

Using the TELL-Seq™ WGS library prep kit you can sequence novel genomes. This works for small bacterial genomes, medium sized insect, plant, and fungi genomes and large animal and plant genomes. The TELL-Seq™ kit will turn an ordinary short-read 2nd generation DNA sequencer into a long-read 3rd generation DNA sequencer. If you really want to understand a new genome, including gene synteny, you must look at long-reads.

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METAGENOMICS SEQUENCING

Samples containing disparate microbes are essential to gaining insight into our ecosystem as well as human disease. The TELL-Seq™ WGS Library Prep kit will allow you to sequence samples at a metagenomic scale; providing long-read like results with the ease of short-read sequencing. To see what is truly in a mixed microbial sample use the power of long-read DNA sequencing with the simplicity of short-read sequencing. 

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STRUCTURAL VARIATION

Short-read DNA sequencers have trouble identifying large structural variants. These could be in the form of copy number variants, translocations, duplications, deletions, insertions, and inversions. The TELL-Seq™ WGS Library Prep kit links short-reads turning them into long-reads. The linked, long-read data that you get using the TELL-Seq™ kit will allow you to take a step back and see what you were missing.

Phasing icon.PNG

WHOLE GENOME PHASING

Knowing which homologous chromosome has which sequence is essential to understanding a genome. Whole Genome Phasing is difficult with 2nd generation, short-read, DNA sequencing technology. Phasing provides haplotype information which is important for understanding complex traits and variant linkages. The TELL-Seq™ WGS DNA Library Prep kit helps with this by linking short-reads to larger fragments and therefore long-reads.


TELL-Seq™ Application Notes


TELL-Seq™ Software

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