qPCRBIO Probe 1-Step Go Separate-ROX

PCR BiosystemsSKU: PCR-PB25.44-01

Title: 100 x 20 ul rxns
Price:
$156

Description

qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and ultra-sensitive probe-based real-time RT-PCR.

The kit is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacon probes.

We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.
The kit includes a 2x mix and can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO Probe 1-Step Go is designed to give rapid and accurate results over a broad range of template concentrations and is ideally suited to the detection of RNA viruses including SARS-CoV-2.

The kit includes a thermostable and extremely active modified MMLV reverse transcriptase (RTase Go) and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products giving highly specific and ultra-sensitive real-time RT-PCR with unrivalled efficiency in multiplex. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market-leading performance with minimal or no optimisation.

 

Features:

  • Rapid and sensitive detection of RNA viruses including SARS-CoV-2
  • Rapid extension rate for early Ct values
  • Market-leading sensitivity – increased limit of detection
  • High efficiency in multiplex reactions
  • Advanced RNase inhibitor
  • Antibody-mediated hot start PCR
  • Compatible with all real-time PCR platforms – standard and fast cycling conditions

 

Applications:

  • COVID-19 / SARS-CoV-2 testing and research
  • Diagnostic real-time PCR
  • Multiplex or singleplex
  • Detection of low copy number targets
  • Absolute quantification
  • Relative gene expression analysis
  • TaqMan®, Scorpions® and molecular beacon probes

 

qPCRBIO Probe 1-Step Go Separate-ROX

 Component  100 Reactions  300 Reactions  1200 Reactions
 2x qPCRBIO Probe 1-Step Go No-ROX  1 x 1mL  3 x 1mL  12 x 1mL
 20x RTase Go with RNase Inhibitor  1 x 100μL  1 x 300μL  4 x 300μL
 50μM ROX Additive  1 x 200μL  1 x 200μL  4 x 200μL

 

Storage:
On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

 

 

 



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